File:WNT3A transfer via filopodia.png

Summary

WNT3A transfer via filopodia

(a-c) Fluorescence photomicrographs of adjacent HeLa cells expressing RUSH-eGFP-WNT3A at indicated time points after biotin addition. From S7 video.

(a) WNT3A is in the Golgi after biotin treatment. Inset showing WNT3A movement along filopodia from one WNT3A-high cell to another WNT3A-low cell (based on GFP intensities). Arrow shows filopodic bridge transporting WNT3A.

(b) At t = 33:40, multiple vesicles are seen on the filopodia.

(c) At t = 40:00.

(d) Fluorescence photomicrographs of HeLa cells (expressing RUSH-mCherry-WNT3A and stained with CellMask Deep Blue membrane dye co-plated with RNF43-mutant HPAF-II cells stained with CellMask Deep Green membrane dye.

From S9 Video (i) Inset showing filopodia extending from HeLa cell to HPAF-II cell at 14:16. (ii-iii) Inset showing WNT3A (mCherry) on the filopodia from HeLa cells moving to HPAF-II cells at indicated time points.

Links: WNT

Reference

Moti N, Yu J, Boncompain G, Perez F & Virshup DM. (2019). Wnt traffic from endoplasmic reticulum to filopodia. PLoS ONE , 14, e0212711. PMID: 30794657 DOI.

Copyright

© 2019 Moti et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

https://doi.org/10.1371/journal.pone.0212711.g005