ACPS Seminar 2014 - Implantation
|Understanding Implantation and the new Uterine Tube Biobank
School of Medical Sciences E: firstname.lastname@example.org
Human Development - Week 1
|<mediaplayer width='500' height='450' image="http://embryology.med.unsw.edu.au/embryology/images/0/07/Human_blastocyst_day_1-5.jpg">File:Human_blastocyst_day_3-6.mp4</mediaplayer>
Human Development - Week 2
- Process of implantation during week 2 (GA week 4) of development in humans.
|<mediaplayer width='250' height='260' image="http://php.med.unsw.edu.au/embryology/images/a/a9/Week2_001_icon.jpg">File:Week2_001.mp4</mediaplayer>||The beginning of the animation shows adplantation to the the uterus lining (endometrium epithelium).
|Section view||Surface view|
Corpus luteum supported by trophoblast secreted hCG.
- Most data from animal models (mouse) of implantation.
- Very little human data for early (first trimester) events.
|Adplantation Signaling |
|Early Implantation Signaling|
|The movie shows an ectopic embryo (less than GA 10 weeks) implanted in the left uterine tube.
|Ectopic GA Week 7||Ectopic GA Week 10|
Image: Dr Steven O'Connor (Houston, Texas)
|First trimester placental villi||Term placental villi|
- Ectopic Model for implantation - some differences (e.g. Endocannabinoids G protein-coupled cannabinoid receptor CB1, ectopic pregnancy show down-regulation)
- Most published human studies are based upon only a very few tissue samples.
- Our Biobank tissues will also be available to other implantation researchers.
- Approval to collect tissue from multiple sites.
Tube Analysis Techniques
- Analysing the proteins expressed at the site of implantation (fetal and maternal).
- Analysing the messenger RNA (mRNA) that encodes protein expression.
- Analysis of microRNA (miRNA) that regulates gene and protein expression.
- Later DNA genetic analysis (are there ectopic implantation associated genes?).
- Expression by fetal cells protects from maternal immune rejection.
HLA-G - Human leukocyte antigen G (HLA-G, HLA-6.0; HLA60, T-CELL A LOCUS; TCA)
- HLA-G is a non-classical HLA class-Ib molecule expressed by the extravillous cytotrophoblasts (EVT) of the placenta.
- 338 AA protein, mRNA differential splicing gives rise to 7 isoforms: 4 membrane-bound forms (HLA-G1, G2, G3, G4) and 3 soluble forms (sHLA-G5, G6, G7).
- miRNA regulated expression.
HLA-G expression (red) on trophoblast cell columns (CC) and extravillous trophoblasts.
- We have established a Uterine Tube Biobank at UNSW.
- First samples collected Sep 2013.
- We now have patient samples from:
- ectopic implantation tube
- control tube, from outside the implantation site
- control tube, from non-pregnant tube.
- Hope to also have control tube from hormonally induced pseudo-pregnacy.
- Three research students (Tatiana, Chi and Liz) working on the project.
- preparing protein, mRNA, miRNA and DNA.
- analysing expression of implantation associated proteins in these tissues.
- First study of a large number of first trimester implantation samples.
- Patients who have donated to this study.
- Prof William Ledger my clinical collaborator.
- Dr Anusha Hettiaratchi the Manager of Lowy Biorepository.
- Dr Tatiana Zandanova, Ngoc Chi Vo and Elizabeth Dalton three research students currently developing the project.
- Australian Centre for Perinatal Science for supporting developmental research and researchers.
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- Prof William Ledger
- Australian Centre for Perinatal Science
- UNSW Biobank
- miRNA microRNA database | MicroRNA
Cite this page: Hill, M.A. (2019, December 12) Embryology ACPS Seminar 2014 - Implantation. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/ACPS_Seminar_2014_-_Implantation
- © Dr Mark Hill 2019, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G