Talk:Bovine Development: Difference between revisions
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Animation http://www.ansi.okstate.edu/resource-room/reprod/all/animations/pregnancy_cow.htm | Animation http://www.ansi.okstate.edu/resource-room/reprod/all/animations/pregnancy_cow.htm | ||
==2010== | |||
===A small set of extra-embryonic genes defines a new landmark for bovine embryo staging=== | |||
Reproduction. 2010 Oct 6. [Epub ahead of print] | |||
Degrelle SA, Le Cao KA, Heyman Y, Everts RE, Campion E, Richard C, Ducroix-Crepy C, Tian C, Lewin H, Renard JP, Robert-Granié C, Hue I. | |||
S Degrelle, UMR Biologie du Developpement et Reproduction, INRA, JOUY EN JOSAS, 78350, France. | |||
Abstract | |||
Axis specification in the mouse is determined by a sequence of reciprocal interactions between embryonic and extra-embryonic tissues so that a few extra-embryonic genes appear as 'patterning' the embryo. Considering these interactions as essential but lacking in most mammals the genetically driven approaches used in the mouse and the corresponding patterning mutants, we examined whether a molecular signature originating from extra-embryonic tissues could relate to the developmental stage of the embryo proper and predict it. To this aim we profiled bovine extra-embryonic tissues at peri-implantation stages, when gastrulation and early neurulation occur, and analysed the subsequent expression profiles through the use of predictive methods as previously reported for tumour classification. A set of 6 genes (CALM1, CPA3, CITED1, DLD, HNRNPDL, TGFB3), half of which had not been previously associated to any extra-embryonic feature, appeared significantly discriminative and mainly dependent on embryonic tissues for its faithful expression. The predictive value of this set of genes for gastrulation and early neurulation stages, as assessed on naïve samples, was remarkably high (93%). In silico connected to the bovine orthologues of the mouse patterning genes, this gene set is proposed as new trait for embryo staging. As such, this will allow saving the bovine embryo proper for molecular or cellular studies. To us, it offers as well new perspectives for developmental phenotyping and modelling of embryonic/extra-embryonic co-differentiation. | |||
PMID: 20926692 | |||
http://www.biolreprod.org/content/79/6/1219.full | http://www.biolreprod.org/content/79/6/1219.full | ||
"The number of morphologically healthy oocytes in the ovaries of mammals is remarkably variable at birth, ranging, for example, from 350 000 to 1 100 000 in humans [1–3] and approximately 14 000 to 250 000 in cattle [4, 5]" | "The number of morphologically healthy oocytes in the ovaries of mammals is remarkably variable at birth, ranging, for example, from 350 000 to 1 100 000 in humans [1–3] and approximately 14 000 to 250 000 in cattle [4, 5]" | ||
Revision as of 13:45, 29 November 2010
Animation http://www.ansi.okstate.edu/resource-room/reprod/all/animations/pregnancy_cow.htm
2010
A small set of extra-embryonic genes defines a new landmark for bovine embryo staging
Reproduction. 2010 Oct 6. [Epub ahead of print]
Degrelle SA, Le Cao KA, Heyman Y, Everts RE, Campion E, Richard C, Ducroix-Crepy C, Tian C, Lewin H, Renard JP, Robert-Granié C, Hue I.
S Degrelle, UMR Biologie du Developpement et Reproduction, INRA, JOUY EN JOSAS, 78350, France. Abstract Axis specification in the mouse is determined by a sequence of reciprocal interactions between embryonic and extra-embryonic tissues so that a few extra-embryonic genes appear as 'patterning' the embryo. Considering these interactions as essential but lacking in most mammals the genetically driven approaches used in the mouse and the corresponding patterning mutants, we examined whether a molecular signature originating from extra-embryonic tissues could relate to the developmental stage of the embryo proper and predict it. To this aim we profiled bovine extra-embryonic tissues at peri-implantation stages, when gastrulation and early neurulation occur, and analysed the subsequent expression profiles through the use of predictive methods as previously reported for tumour classification. A set of 6 genes (CALM1, CPA3, CITED1, DLD, HNRNPDL, TGFB3), half of which had not been previously associated to any extra-embryonic feature, appeared significantly discriminative and mainly dependent on embryonic tissues for its faithful expression. The predictive value of this set of genes for gastrulation and early neurulation stages, as assessed on naïve samples, was remarkably high (93%). In silico connected to the bovine orthologues of the mouse patterning genes, this gene set is proposed as new trait for embryo staging. As such, this will allow saving the bovine embryo proper for molecular or cellular studies. To us, it offers as well new perspectives for developmental phenotyping and modelling of embryonic/extra-embryonic co-differentiation.
PMID: 20926692
http://www.biolreprod.org/content/79/6/1219.full
"The number of morphologically healthy oocytes in the ovaries of mammals is remarkably variable at birth, ranging, for example, from 350 000 to 1 100 000 in humans [1–3] and approximately 14 000 to 250 000 in cattle [4, 5]"
