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- 08:34, 16 June 2012 Z8600021 talk contribs uploaded File:Mouse zygote mitosis metaphase.jpg (==Mouse Zygote Mitosis Metaphase== Mouse zygotes male pronucleus contains 5-hydroxymethylcytosine (5hmC) formed by enzymatic oxidation of 5-methylcytosine (5mC). 5mC and 5hmC staining reveal two separate chromosome sets at metaphase of zygote division. A)