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- 12:28, 15 December 2010 S8600021 talk contribs uploaded File:Blastomere mitotic spindle orientation.jpg (==Blastomere Mitotic Spindle Orientation== Determination of spindle poles and embryo centre coordinates For a given spindle, the position of three points were determined, by moving through the stack of images (z1, z2, z3, …). The value of the α angle )