Movie - Mitosis 1

From Embryology
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File:Mitosis 01 icon.jpg</wikiflv> Corresponds to Fig. 2 A. A time-lapse video sequence of an EGFP-topoII-expressing GT2-LPk cell imaged from late prometaphase through telophase.

Original File Name: 5 Mb (see also Quicktime version)

Chromosomes align at the metaphase plate with EGFP-topoII concentrated at kinetochores and along the axes of the chromosome arms.

As chromosomes separate at anaphase, the kinetochore concentration disappears.

In late anaphase, the levels of EGFP-topo II rise inthe cytoplasm, with some concentrated into foci that are motile and, at telophase, appear to fuse with the reforming nuclei.

A phase contrast image of the two daughter cells is shown at the end of the sequence.

The fluorescence images in this sequence were individually scaled in order to optimize visualization of the EGFP-topoII cytoplasmic foci that appear inanaphase and telophase.



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