Mouse Fertilization Movie

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<mediaplayer width='500' height='500' image="">File:Fertilization 001.mp4</mediaplayer>

Fertilization of the Mouse Oocyte

  • White arrowhead - sperm
  • White exclamation mark - (at beginning of movie) time of fertilization (short cytoplasmic contraction)
  • Black asterisk - site of second polar body formation
  • White asterisk - site of fertilization cone formation

Links: Quicktime | Quicktime version | Flash version | Images from Movie | Fertilization | Mouse Development

Mouse oocyte fertilization 01.jpg

Sequential differential interference contrast (DIC) images of mouse embryos during in vitro fertilisation

  1. Spermatozoa penetrates the zone pellucida (ZP).
  2. Spermatozoa swims around the perivitelline space (PVS).
  3. Spermatozoa swims around the perivitelline space (PVS).
  4. Spermatozoa hits the first polar body (1pb).
  5. Spermatozoa binds the oocyte membrane.
  6. Second polar body (2pb) extrudes and the fertilization cone forms.


  • White arrowheads - indicate the sperm
  • Red arrowheads - first polar body (1pb)
  • Yellow arrowheads - the collapsing first polar body (1pb).
  • White and red asterisks - mark the fertilization cone and the 2pb, respectively.

Scale bar represent 20 μm.


<pubmed>16620153</pubmed>| PMC1440932 | PLoS Biol.

Citation: Motosugi N, Dietrich J-E, Polanski Z, Solter D, Hiiragi T (2006) Space Asymmetry Directs Preferential Sperm Entry in the Absence of Polarity in the Mouse Oocyte. PLoS Biol 4(5): e135. doi:10.1371/journal.pbio.0040135


© 2006 Motosugi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.