File talk:Mouse CT axes E11.5.jpg
Mouse Embryo Computed Tomography
Isosurface of E11.5 embryos scanned at 8-μm resolution.
Comparison of Virtual and Paraffin Histology of an E11.5 Embryo Scanned at 8 μm
- (A) Isosurface renderings of the CT-scanned embryo.
- (B) Maximum intensity projection of the same embryo, with overlying places of section.
- (C–E) Sagittal, coronal, and axial sections of an E11.5 littermate.
- (F–H) Sagittal, coronal, and axial computed tomography sections of the embryo in panels (A) and (B), corresponding to the planes of section in panels (C–E). At low-power magnification, virtual and true paraffin histology demonstrate a similar level of detail. Scale bar indicates 400 μm.
a, cardiac atrium; cv, cardinal vein; drg, dorsal root ganglia; fv, forebrain vesicle; liv, liver; nt, neural tube; v, cardiac ventricle; v4, fourth ventricle.
doi:10.1371/journal.pgen.0020061.g001
Original File Name:journal.pgen.0020061.g001.tif
<pubmed>16683035</pubmed>| PLoS Genetics
Citation: Johnson JT, Hansen MS, Wu I, Healy LJ, Johnson CR, et al. (2006) Virtual Histology of Transgenic Mouse Embryos for High-Throughput Phenotyping. PLoS Genet 2(4): e61. doi:10.1371/journal.pgen.0020061
Editor: Wayne Frankel, The Jackson Laboratory, United States of America
Received: January 23, 2006; Accepted: March 13, 2006; Published: April 28, 2006
Copyright: © 2006 Johnson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.