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Image title	In vitro fertilization results with control (A-D) and MT- spermatozoa (E-H).Oocytes fertilized using control spermatozoa showed proper fertilization (PF) as judged by the presence of both polar bodies (PBs) and both pronuclei [PN].(A-D). Oocytes fertilized with MT-spermatozoa showed defective fertilization. In these spermatozoa, only meiotic spindle reorganization was visible (asterisk, E-H) and the 2nd polar body extrusion had failed (red arrow in E-H). Oocytes were stained with Hoechst 33342 to visualize the polar bodies and pronuclei and the images presented are a merge of both brightfield and fluorescence. Magnification used was 400 x. Scale bars indicate 5µm.
Copyright holder	Creative Commons Attribution License
Date(s)	22 May 2014
Publisher	Public Library of Science
Short title	Figure 2
Source	info:doi/10.1371/journal.pone.0097916
Identifier	info:doi/info:doi/10.1371/journal.pone.0097916.g002
IIM version	2