File:Syrian Hamster In Vitro Fertilisation PMID- 24852961.jpeg

PMID: 24852961

Syrian Hamster In Vitro Fertilisation PMID-24852961.jpeg

Oocytes fertilized using control spermatozoa showed proper fertilization (PF) as judged by the presence of both polar bodies (PBs) and both pronuclei [PN].(A-D). Oocytes fertilized with MT-spermatozoa showed defective fertilization. In these spermatozoa, only meiotic spindle reorganization was visible (asterisk, E-H) and the 2nd polar body extrusion had failed (red arrow in E-H). Oocytes were stained with Hoechst 33342 to visualize the polar bodies and pronuclei and the images presented are a merge of both brightfield and fluorescence. Magnification used was 400 x. Scale bars indicate 5 µm.

(Original Figure Legend, image based on data from Archana, Subbarayalu, Purnima, Durgesh, Duvurri, and Sisinthy PMID 24852961)

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<pubmed>24852961</pubmed>

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