File:Spleen structure 01.jpg: Difference between revisions
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Schematic representation of the organization of the spleen (left panel). | Schematic representation of the organization of the spleen (left panel). | ||
* The white pulp consists of T cell zones (also known as the periarteriolar lymphoid sheath (PALS)) containing networks of fibroblastic reticular cells (FRC) surrounding a central arteriole, together with B cell follicles containing a central network of follicular dendritic cells (FDC). | * The white pulp consists of T cell (lymphocyte) zones (also known as the periarteriolar lymphoid sheath (PALS)) containing networks of fibroblastic reticular cells (FRC) surrounding a central arteriole, together with B cell follicles containing a central network of follicular dendritic cells (FDC). | ||
* Marginal zones (MZ) surrounding the white pulp contain marginal reticular cells (MRC), particularly at the edges of the B cell follicles. | * Marginal zones (MZ) surrounding the white pulp contain marginal reticular cells (MRC), particularly at the edges of the B cell follicles. | ||
* Blood and leukocytes entering the spleen pass through branches of the central arteriole, which end in the marginal sinuses and red pulp. | * Blood and leukocytes entering the spleen pass through branches of the central arteriole, which end in the marginal sinuses and red pulp. | ||
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* Immune cells enter the white pulp at regions where the T cell zones abut the MZ, known as the MZ bridging channels. | * Immune cells enter the white pulp at regions where the T cell zones abut the MZ, known as the MZ bridging channels. | ||
An image of a section of mouse spleen generated using multicolour immunofluoresence microscopy illustrates the organization of the white pulp, red pulp, and MZ (centre panel). The distribution of | An image of a section of mouse spleen generated using multicolour immunofluoresence microscopy illustrates the organization of the white pulp, red pulp, and MZ (centre panel). | ||
* The distribution of cells: | |||
** '''T cells''' - (white) CD3+ | |||
** '''B cells''' - (blue) B220+ | |||
** '''macrophages''' - (cyan) CD169+ MZ | |||
** '''dendritic cells''' - (green) (DCs) CD11c+ | |||
** '''stromal cells''' - (red) ER-TR7+ | |||
* The distinct organization of stromal cells in different regions of the spleen is shown by single-colour immunofluoresence staining (right panel). | |||
* Networks of stromal cells and reticular fibres form in the white pulp, including the fibroblastic reticular cells (FRCs) in T cell zones, follicular dendritic cells (FDCs) in B cell follicles (ER-TR7−) and marginal reticular cells (MRCs) in the MZ. | |||
* A dense network of stromal cells and reticular fibres is present in the red pulp. | |||
Scale bars represent 130 μM. | |||
Revision as of 12:56, 26 February 2012
Spleen Structure
Schematic representation of the organization of the spleen (left panel).
- The white pulp consists of T cell (lymphocyte) zones (also known as the periarteriolar lymphoid sheath (PALS)) containing networks of fibroblastic reticular cells (FRC) surrounding a central arteriole, together with B cell follicles containing a central network of follicular dendritic cells (FDC).
- Marginal zones (MZ) surrounding the white pulp contain marginal reticular cells (MRC), particularly at the edges of the B cell follicles.
- Blood and leukocytes entering the spleen pass through branches of the central arteriole, which end in the marginal sinuses and red pulp.
- In the cords of the red pulp, a dense network of reticular fibroblasts and fibres construct an open blood network, which is marked by its lack of a typical endothelial cell lining.
- Large numbers of macrophages phagocytose dying or damaged red blood cells in the red pulp (not shown).
- Immune cells enter the white pulp at regions where the T cell zones abut the MZ, known as the MZ bridging channels.
An image of a section of mouse spleen generated using multicolour immunofluoresence microscopy illustrates the organization of the white pulp, red pulp, and MZ (centre panel).
- The distribution of cells:
- T cells - (white) CD3+
- B cells - (blue) B220+
- macrophages - (cyan) CD169+ MZ
- dendritic cells - (green) (DCs) CD11c+
- stromal cells - (red) ER-TR7+
- The distinct organization of stromal cells in different regions of the spleen is shown by single-colour immunofluoresence staining (right panel).
- Networks of stromal cells and reticular fibres form in the white pulp, including the fibroblastic reticular cells (FRCs) in T cell zones, follicular dendritic cells (FDCs) in B cell follicles (ER-TR7−) and marginal reticular cells (MRCs) in the MZ.
- A dense network of stromal cells and reticular fibres is present in the red pulp.
Scale bars represent 130 μM.
Reference
<pubmed>19644499</pubmed>| PMC2785037 | Nat Rev Immunol.
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