File:Schematic representation of the various treatment windows and experimental design..jpg

Schematic representation of the various treatment windows and experimental design

Also indicated in blue are testis differentiation in the rat (at ~embryonic day (e)13.5), reproductive tract differentiation (from ~e18.5 onwards) and the masculinization programming window (MPW, e15.5–e18.5), during which anogenital distance (AGD) is programmed. Three treatment windows were used in this study, namely ‘full treatment window’ (e13.5–e20.5), ‘MPW treatment window’ (e15.5–e18.5) and ‘late treatment window’ (e19.5–e20.5). At e21.5 embryos were isolated. For each embryo, AGD was measured, testes were dissected and weighed, before 1 testis was fixed for subsequent immunohistochemistry (IHC) and 1 testis was frozen on dry ice for subsequent intratesticular testosterone (ITT) measurement. In a separate experiment, pregnant rats were treated from e13.5–e21.5 and pups were collected on postnatal day (pnd) 8 and the same procedures as for the fetal samples were undertaken.

Reference

<pubmed>22253897</pubmed>

Copyright

© 2012 van den Driesche et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

--Mark Hill (talk) 10:21, 7 November 2014 (EST) Assessment - Figure relates to project topic contains reference, copyright and student template. The file name and description makes no sense, "treatment windows and experimental design"? This should have been better identified and named.

Note - This image was originally uploaded as part of an undergraduate science student project and may contain inaccuracies in either description or acknowledgements. Students have been advised in writing concerning the reuse of content and may accidentally have misunderstood the original terms of use. If image reuse on this non-commercial educational site infringes your existing copyright, please contact the site editor for immediate removal.