File:Rat prostate epithelial branching.jpg

Rat Prostate (whole-mount) Wnt3a and DKK1 regulate prostatic epithelial branching morphogenesis

Whole mount ventral prostates were prepared from P2 rats and maintained for 7 days in serum-free medium in the

(A) serum-free medium

(B) presence of 50 nM of Wnt3a

(C) presence of 400 nM of DKK1

Similar patterns were consistently seen in 3 repeat experiments of 5–6 prostate organs per group in each individual experiment. Note that addition of either Wnt3a or DKK1 to the culture resulted a change in epithelial branching morphogenesis. Quantification of the cultures by measuring the diameter of the cultured prostates (D) and the ductal tips (E) using Axiovision software and the branching points (F) were done by analyzing 4 randomly selected cultures per group. Data are expressed as mean + SEM (t-test, compared to control cultures).

Bar, 400 µm.

Original file name: Figure 1. Journal.pone.0002186.g001.png

Reference

Citation: Wang B-E, Wang X-D, Ernst JA, Polakis P, Gao W-Q (2008) Regulation of Epithelial Branching Morphogenesis and Cancer Cell Growth of the Prostate by Wnt Signaling. PLoS ONE 3(5): e2186. doi:10.1371/journal.pone.0002186

Editor: Hernan Lopez-Schier, Centre de Regulacio Genomica, Spain

Received: January 17, 2008; Accepted: April 7, 2008; Published: May 14, 2008

Copyright: © 2008 Wang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.