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Organoid establishment from stem cells and cancer cells

Embryonic stem cells from human embryonic tissues and induced pluripotent stem cells from adult tissues firstly experience directed differentiation, generate floating spheroids, and subsequently are planted on extracellular matrix in specific culture medium to initiate organoid culture.

Primary tissues from patients can be dissociated into functional units, which contain somatic stem cells. These somatic stem cells are enriched and cultured in three-dimensional medium to form organoids. Tumor cells isolated from cancer tissues can also form tumoroids in well-defined three-dimensional culture.

Links: organoids

Reference

Xu H, Lyu X, Yi M, Zhao W, Song Y & Wu K. (2018). Organoid technology and applications in cancer research. J Hematol Oncol , 11, 116. PMID: 30219074 DOI.

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Cite this page: Hill, M.A. (2024, April 27) Embryology Organoids overview 01.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Organoids_overview_01.jpg

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© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G