File:Mouse tooth movie 02.mp4
Mouse_tooth_movie_02.mp4 (file size: 17.91 MB, MIME type: video/mp4)
S2 Video. Ex vivo live imaging of a developing tooth germ.
embryonic days (E) 12.5–13.5 mice.
Time-lapse imaging of a developing tooth germ derived from a transgenic mouse embryo co-expressing Fucci-G0/G1 probe and H2BEGFP. The tooth germ was cultured for 5 days ex vivo, and the images were taken at 30-min intervals. The lingual side is on the left. Also see Fig 1E. https://doi.org/10.1371/journal.pone.0161336.s010 (MP4)
To visualize these tooth morphogenetic dynamics sequentially, we established an ex vivo time-lapse imaging system using transgenic mouse embryos co-expressing the Fucci-G0/G1 probe (Cdt1-mKO) and enhanced green fluorescent protein-labeled human histone 2B (H2B-EGFP; S1 and S2 Videos).
Morita R, Kihira M, Nakatsu Y, Nomoto Y, Ogawa M, Ohashi K, et al. (2016) Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations. PLoS ONE 11(9): e0161336. https://doi.org/10.1371/journal.pone.0161336
Copyright: © 2016 Morita et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0161336
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current | 14:32, 1 August 2017 | (17.91 MB) | Z8600021 (talk | contribs) | S2 Video. Ex vivo live imaging of a developing tooth germ. embryonic days (E) 12.5–13.5 mice. Time-lapse imaging of a developing tooth germ derived from a transgenic mouse embryo co-expressing Fucci-G0/G1 probe and H2BEGFP. The tooth germ was cultu... |
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