File:Mouse spleen capsulin expression.jpg

Embryonic expression pattern of lacZ from targeted capsulin allele

Embryos homozygous for the capsulin-lacZ knockin allele were stained for lacZ expression, sectioned, and stained with hematoxylin/eosin for histologic analysis.

A and E show whole-mount embryos at E9.5 and E13.5, respectively. The vertical line in A points to the septum transversum (st).

(B) Transverse section through the branchial arches at E9.5 showing a core of lacZ-positive cells, representing skeletal muscle progenitors.

(C) Transverse section through an E10.5 embryo at the level of the posterior region of the heart. LacZ staining can be seen in the region of the septum transversum (st) below the heart and in the mesothelium along the pleural cavity (p), adjacent to the future lung buds.

(D) Sagittal section through an E10.5 embryo showing lacZ staining within the proepicardial organ (po) and epicardium. b, branchial arch.

(F) Transverse section through an E10.5 embryo more posterior than in C, showing asymmetric lacZ staining in visceral mesothelium and adjacent mesoderm on the left side of the embryo and adjacent to the gut (g), representing the splenic anlage (indicated by arrow).

(G) Transverse section at E11.5 through the dorsolateral region of the stomach (st) and splenic primordium, showing asymmetric left-sided expression of lacZ (indicated by arrow). l, liver.

(H) Transverse section at E10.5 showing lacZ staining in the lung primordium (l), epicardium (e), and condensing metanephric mesenchyme (m).

Original file name: Figure 3. http://www.pnas.org/content/97/17/9525/F3.large.jpg

Reference

Lu J, Chang P, Richardson JA, Gan L, Weiler H & Olson EN. (2000). The basic helix-loop-helix transcription factor capsulin controls spleen organogenesis. Proc. Natl. Acad. Sci. U.S.A. , 97, 9525-30. PMID: 10944221

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Proceedings National Academy of Sciences (PNAS) Liberalization of PNAS copyright policy: Noncommercial use freely allowed Note original Author should be contacted for permission to reuse for Educational purposes. See also PNAS Author Rights and Permission FAQs

Cozzarelli NR, Fulton KR, Sullenberger DM. Liberalization of PNAS copyright policy: noncommercial use freely allowed. Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12399. PMID15314225 "Our guiding principle is that, while PNAS retains copyright, anyone can make noncommercial use of work in PNAS without asking our permission, provided that the original source is cited."

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