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Comparison of Spry1, Spry2, and Wnt reporter expression domains from pan-placodal region to otic placed stages

All cranial placodes, including the otic placode, originate from the pan-placodal region (PPR)

In situ hybridization analysis of Spry1 and Spry2 expression domains compared to Wnt reporter activity in TCF/Lef-lacZ embryos at the stages indicated. Transverse sections are shown, dorsal oriented to the top. a – cSpry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity at early somite stages in the posterior PPR. Arrowhead indicates the presumptive otic/epibranchial region. Little or no TCF/Lef-lacZ reporter activity is detected in the posterior PPR at this stage (c). (d – f’) Spry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity in anterior (d – f) and posterior (d’ – f’) transverse sections through the OEPD. The entire OEPD is bracketed. (g – i”) Spry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity in anterior (g – i), medial (g’ – i’), and posterior (g” – i”) transverse sections through the otic placode (bracketed).

Abbreviations: Sprouty1 (Spry1), Sprouty2 (Spry2), pan-placodal region (PPR), otic-epibranchial progenitor domain (OEPD), neural ectoderm (ne), endoderm (ed), hindbrain (hb). Scale bar, 50 μm

Links: Inner Ear Development | Mouse Development

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<pubmed>6443994</pubmed>| BMC Developmental Biology

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© 2015 Wright et al. Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Wright et al. BMC Developmental Biology 2015 15:33 doi:10.1186/s12861-015-0083-8 S12861-015-0083-8-1.jpg

Cite this page: Hill, M.A. (2024, May 14) Embryology Mouse otic placode gene expression 01.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Mouse_otic_placode_gene_expression_01.jpg

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	Date/Time	Thumbnail	Dimensions	User	Comment
current	09:15, 27 October 2015		358 × 677 (86 KB)	Z8600021 (talk \| contribs)	==Comparison of Spry1, Spry2, and Wnt reporter expression domains from PPR to otic placed stages== In situ hybridization analysis of Spry1 and Spry2 expression domains compared to Wnt reporter activity in TCF/Lef-lacZ embryos at the stages indicated....

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Hearing - Inner Ear Development

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@@ Line 1: / Line 1: @@
-==Comparison of Spry1, Spry2, and Wnt reporter expression domains from PPR to otic placed stages==
+==Comparison of Spry1, Spry2, and Wnt reporter expression domains from pan-placodal region to otic placed stages==
-In situ hybridization analysis of Spry1 and Spry2 expression domains compared to Wnt reporter activity in TCF/Lef-lacZ embryos at the stages indicated. Transverse sections are shown, dorsal oriented to the top. a – cSpry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity at early somite stages in the posterior PPR. Arrowhead indicates the presumptive otic/epibranchial region. Little or no TCF/Lef-lacZ reporter activity is detected in the posterior PPR at this stage (c). (d – f’) Spry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity in anterior (d – f) and posterior (d’ – f’) transverse sections through the OEPD. The entire OEPD is bracketed. (g – i”) Spry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity in anterior (g – i), medial (g’ – i’), and posterior (g” – i”) transverse sections through the otic placode (bracketed). Abbreviations: neural ectoderm (ne), endoderm (ed), hindbrain (hb). Scale bar, 50 μm
+All cranial placodes, including the otic placode, originate from the pan-placodal region (PPR)
+In situ hybridization analysis of Spry1 and Spry2 expression domains compared to Wnt reporter activity in TCF/Lef-lacZ embryos at the stages indicated. Transverse sections are shown, dorsal oriented to the top. a – cSpry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity at early somite stages in the posterior PPR. Arrowhead indicates the presumptive otic/epibranchial region. Little or no TCF/Lef-lacZ reporter activity is detected in the posterior PPR at this stage (c). (d – f’) Spry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity in anterior (d – f) and posterior (d’ – f’) transverse sections through the OEPD. The entire OEPD is bracketed. (g – i”) Spry1 expression, Spry2 expression, and TCF/Lef-lacZ reporter activity in anterior (g – i), medial (g’ – i’), and posterior (g” – i”) transverse sections through the otic placode (bracketed).
+Abbreviations: Sprouty1 (Spry1), Sprouty2 (Spry2), pan-placodal region (PPR), otic-epibranchial progenitor domain (OEPD), neural ectoderm (ne), endoderm (ed), hindbrain (hb).  Scale bar, 50 μm
+:'''Links:''' [[Hearing_-_Inner_Ear_Development|Inner Ear Development]] | [[Mouse Development]]
 ===Reference===
@@ Line 12: / Line 19: @@
-Wright et al. BMC Developmental Biology 2015 15:33   doi:10.1186/s12861-015-0083-8
+Wright et al. BMC Developmental Biology 2015 15:33   doi:10.1186/s12861-015-0083-8 S12861-015-0083-8-1.jpg
-S12861-015-0083-8-1.jpg
-BMC Dev Biol. 2015 Oct 6;15(1):33. doi: 10.1186/s12861-015-0083-8.
+{{Footer}}
-Cooperative and independent functions of FGF and Wnt signaling during early inner ear development.
+[[Category:Mouse]] [[Category:Hearing]] [[Category:Placode]]  [[Category:Inner Ear]]
-Wright KD1, Mahoney Rogers AA2, Zhang J3, Shim K4.
-Author information
-Abstract
-BACKGROUND:
-In multiple vertebrate organisms, including chick, Xenopus, and zebrafish, Fibroblast Growth Factor (FGF) and Wnt signaling cooperate during formation of the otic placode. However, in the mouse, although FGF signaling induces Wnt8a expression during induction of the otic placode, it is unclear whether these two signaling pathways functionally cooperate. Sprouty (Spry) genes encode intracellular antagonists of receptor tyrosine kinase signaling, including FGF signaling. We previously demonstrated that the Sprouty1 (Spry1) and Sprouty2 (Spry2) genes antagonize FGF signaling during induction of the otic placode. Here, we investigate cross talk between FGF/SPRY and Wnt signaling during otic placode induction and assess whether these two signaling pathways functionally cooperate during early inner ear development in the mouse.
-METHODS:
-Embryos were generated carrying combinations of a Spry1 null allele, Spry2 null allele, β-catenin null allele, or a Wnt reporter transgene. Otic phenotypes were assessed by in situ hybridization, semi-quantitative reverse transcriptase PCR, immunohistochemistry, and morphometric analysis of sectioned tissue.
-RESULTS:
-Comparison of Spry1, Spry2, and Wnt reporter expression in pre-otic and otic placode cells indicates that FGF signaling precedes and is active in more cells than Wnt signaling. We provide in vivo evidence that FGF signaling activates the Wnt signaling pathway upstream of TCF/Lef transcriptional activation. FGF regulation of Wnt signaling is functional, since early inner ear defects in Spry1 and Spry2 compound mutant embryos can be genetically rescued by reducing the activity of the Wnt signaling pathway. Interestingly, we find that although the entire otic placode increases in size in Spry1 and Spry2 compound mutant embryos, the size of the Wnt-reporter-positive domain does not increase to the same extent as the Wnt-reporter-negative domain.
-CONCLUSIONS:
-This study provides genetic evidence that FGF and Wnt signaling cooperate during early inner ear development in the mouse. Furthermore, our data suggest that although specification of the otic placode may be globally regulated by FGF signaling, otic specification of cells in which both FGF and Wnt signaling are active may be more tightly regulated.
-PMID 26443994