File:Mouse E9.5 neural crest beta actin KO.jpg
Original file (2,722 × 2,573 pixels, file size: 732 KB, MIME type: image/jpeg)
Mouse E9.5 Beta Actin Knock Out embryos ( Actb−/−) show Defects in Neural Crest Ontogeny
- A - Crabp1 *cellular retinoic acid binding protein 1) whole mount and section ISH (at the indicated planes: a, b, c and d) on Actb+/+ and Actb−/− embryos. For the latter all sections show absence of neural crest cells in certain regions of the sections. Actb−/− neural crest cells are not able to reach the brachial arches (arrows in section d).
- B - Sox10 whole mount embryo ISH marks the migrating neural crest. Sox10 ISH shows comparable results as the Crabp1 probe.
- C - Pax3 marks the dermomyotome and the cranial neural crest. Compared to Actb+/+ embryos, the Actb−/− embryos show a similar expression pattern of pax3 at the level of the dermomyotomes, but a reduction in the cranial region. Panels depicting Actb+/+ and Actb−/− embryos are of the same magnification.
- Links; Neural Crest Development | Retinoic acid | Sox | Pax | Mouse Development
Reference
<pubmed>24409333</pubmed>| PLoS One.
Copyright
© 2014 Tondeleir et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Beta-actin is required for proper mouse neural crest ontogeny. Tondeleir D, Noelanders R, Bakkali K, Ampe C. PLoS One. 2014 Jan 7;9(1):e85608. doi: 10.1371/journal.pone.0085608. eCollection 2014. PMID 24409333
doi:10.1371/journal.pone.0085608.g002 Journal.pone.0085608.g002.jpg Original figure relabelled, text above from figure legend.
Cite this page: Hill, M.A. (2024, April 27) Embryology Mouse E9.5 neural crest beta actin KO.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Mouse_E9.5_neural_crest_beta_actin_KO.jpg
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G
File history
Click on a date/time to view the file as it appeared at that time.
| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 10:53, 9 September 2015 | | 2,722 × 2,573 (732 KB) | Z8600021 (talk | contribs) | Figure 2. At E9.5 Actb−/− embryos show defects in neural crest ontogeny. (A) Crabp1 whole mount and section ISH (at the indicated planes: a, b, c and d) on Actb+/+ and Actb−/− embryos. For the latter all sections show absence of neural crest ce... |
You cannot overwrite this file.
File usage
There are no pages that use this file.
