File:Mouse CT E10.5 head.jpg: Difference between revisions
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Original File name: 1471-213X-10-18-2-l.jpg http://www.biomedcentral.com/1471-213X/10/18/figure/F2 | Original File name: 1471-213X-10-18-2-l.jpg http://www.biomedcentral.com/1471-213X/10/18/figure/F2 | ||
==Reference== | |||
<ref name="PMID20163731"><pubmed>20163731</pubmed>|[http://www.biomedcentral.com/1471-213X/10/18 BMC Developmental Biology]</ref> | |||
© 2010 Schmidt et al; licensee BioMed Central Ltd. | |||
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |||
===Reference== | ===Reference== |
Revision as of 11:46, 17 August 2010
Mouse Embryo Computed Tomography
Identically scaled renderings representing 3D μCT scans of embryos (E10.5 head according to paper methods) subjected to different fixation and scanning procedures.
- A - 4% formaldehyde and secondary Bouin's fixation.
- B - 4% formaldehyde + 1% glutaraldehyde with Iothalamate meglumine used as a contrast agent.
- C - 4% formaldehyde + 5% glutaraldehyde plus contrast agent.
All embryos are shown in right 3/4 inferolateral view.
- "Typical μCT renderings are represented by the embryos depicted in Figure 2A-C. The combination of aldehyde fixation and Cysto Conray® yields images (Figure 2B-C) with a surface quality comparable to embryos fixed with Bouin's solution (Figure 2A). Bouin's-fixed embryos appear have a rougher surface texture compared to those fixed with either glutaraldehyde mixture (Figure 2B-C). The identically scaled images of Bouin's fixed embryos appeared generally smaller compared to those fixed with glutaraldehyde mixtures, but a comparison of μCT images only cannot indicate the relative roles of fixation-induced tissue shrinkage or swelling."
Original File name: 1471-213X-10-18-2-l.jpg http://www.biomedcentral.com/1471-213X/10/18/figure/F2
Reference
© 2010 Schmidt et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
=Reference
<pubmed>20163731</pubmed>|BMC Developmental Biology
© 2010 Schmidt et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- ↑ <pubmed>20163731</pubmed>|BMC Developmental Biology
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