File:Mouse-adrenal gland E16.5.jpg

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Effects of Dicer1 ablation on adrenal development in mice

A mouse model, in which the Dicer1 gene was inactivated in the progenitor cells of the adrenals and gonads (by targeting the Steroidogenic factor 1-positive cells), enables the investigation of the function of Dicer1 in the development of the adrenal. Loss of Dicer1 does not impede proper differentiation of the cortex (magenta) and adrenal (green) at 16.5 dpc (shown here), but by 18.5 dpc the adrenals of the Dicer1 conditional knockout are significantly smaller than those in the wild-type. Immunofluorescence was performed by staining for a marker for the adrenal cortex, SF1 (magenta), a marker for the medulla, tyrosine hydroxylase, TH (green) and nuclei (blue - stained with DAPI).

Reference

Huang CC & Yao HH. (2010). Inactivation of Dicer1 in Steroidogenic factor 1-positive cells reveals tissue-specific requirement for Dicer1 in adrenal, testis, and ovary. BMC Dev. Biol. , 10, 66. PMID: 20540774 DOI.

Copyright

© 2010 Huang and Yao; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Original File name: 201007large.jpg http://www.biomedcentral.com/bmcdevbiol/imageofthemonth/2010/07

"Dicer1 is dispensable for formation and differentiation of fetal tissues derived from the SF1-positive adrenogonadal primordium. Dicer1 is essential for maintaining cell survival in adrenal and testis; however, development of the ovary from fetal stages to postnatal day 5 does not require the presence of Dicer1. Our results reveal a tissue-specific requirement of Dicer1 and microRNAs. Future research is needed to understand how the tissue-specific role of Dicer1 is established."

Cite this page: Hill, M.A. (2024, April 20) Embryology Mouse-adrenal gland E16.5.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Mouse-adrenal_gland_E16.5.jpg

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© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G

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current11:05, 28 September 2010Thumbnail for version as of 11:05, 28 September 2010600 × 463 (93 KB)S8600021 (talk | contribs)
10:59, 28 September 2010Thumbnail for version as of 10:59, 28 September 2010600 × 463 (298 KB)S8600021 (talk | contribs)==Effects of Dicer1 ablation on adrenal development in mice== A mouse model, in which the Dicer1 gene was inactivated in the progenitor cells of the adrenals and gonads (by targeting the Steroidogenic factor 1-positive cells), enables the investigation o

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