File:Mouse- forelimb bud Shh expression and BMP pathway.jpg: Difference between revisions

Shh expression and BMP pathway activity in forelimb buds

Excerpt from Figure 6. showing only the normal limb gene expression.

(A) No Shh expression is detected in the posterior mesenchyme of H2Δ/Δc and H2Δ/ΔcGli3Xt/Xt limb buds at E10.25 (32–33 somites).

(B) Bmp4, Grem1, and Msx2 expression at E10.5 (34–35 somites). Note that Grem1 expression is activated, but not up-regulated and expanded distal-anterior in H2Δ/Δc limb buds. In contrast, the Grem1 expression domain appears rather uniform in the majority of all H2Δ/ΔcGli3Xt/Xt limb buds.

(C) Q–PCR quantitation of Shh, Bmp4, Grem1 and Msx2 expression in single limb buds of mouse embryos of the indicated genotypes at ~E10.5 (34–37 somites). Boxes show the average (± standard deviation), dots indicate levels in individual limb bud determined by triplicate analysis. The vertical axis indicates expression levels in percentages of wild-type levels (wild-type average set at 100%). Wt: wild-type (n = 8 single limb buds analyzed); H2: H2Δ/Δc (n = 8); Gli3: Gli3Xt/Xt (n = 7); H2Gli3: H2Δ/ΔcGli3Xt/Xt (n = 6).

All differences discussed in the text are statistically highly significant (p-values between p<0.001 and p<0.05 using Mann-Whitney tests).

Original File name: journal.pgen.1000901.g003.png

Reference

<pubmed>20386744</pubmed>

Distinct roles of Hand2 in initiating polarity and posterior Shh expression during the onset of mouse limb bud development. Galli A, Robay D, Osterwalder M, Bao X, Bénazet JD, Tariq M, Paro R, Mackem S, Zeller R. PLoS Genet. 2010 Apr 8;6(4):e1000901. PMID: 20386744

Citation: Galli A, Robay D, Osterwalder M, Bao X, Bénazet J-D, et al. (2010) Distinct Roles of Hand2 in Initiating Polarity and Posterior Shh Expression during the Onset of Mouse Limb Bud Development. PLoS Genet 6(4): e1000901. doi:10.1371/journal.pgen.1000901

Editor: Clifford J. Tabin, Harvard Medical School, United States of America

Received: September 11, 2009; Accepted: March 9, 2010; Published: April 8, 2010

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