File:Mouse-E11.5.png

Comparison of Virtual and Paraffin Histology of an E11.5 Embryo Scanned at 8 μm

(A) Isosurface renderings of the CT-scanned embryo.

(B) Maximum intensity projection of the same embryo, with overlying places of section.

(C–E) Sagittal, coronal, and axial sections of an E11.5 littermate.

(F–H) Sagittal, coronal, and axial computed tomography sections of the embryo in panels (A) and (B), corresponding to the planes of section in panels (C–E). At low-power magnification, virtual and true paraffin histology demonstrate a similar level of detail. Scale bar indicates 400 μm.

a, cardiac atrium; cv, cardinal vein; drg, dorsal root ganglia; fv, forebrain vesicle; liv, liver; nt, neural tube; v, cardiac ventricle; v4, fourth ventricle.

Reference

PLoS Genet

Citation: Johnson JT, Hansen MS, Wu I, Healy LJ, Johnson CR, et al. (2006) Virtual Histology of Transgenic Mouse Embryos for High-Throughput Phenotyping. PLoS Genet 2(4): e61. doi:10.1371/journal.pgen.0020061

Editor: Wayne Frankel, The Jackson Laboratory, United States of America

Received: January 23, 2006; Accepted: March 13, 2006; Published: April 28, 2006

Copyright: © 2006 Johnson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Original File Name: Journal.pgen.0020061.g001.png