File:Model of Dazl germ cell function.jpg

Model Links Dazl Between Pluripotency, Reprogramming and Differentiation

Green signifies Oct4ΔPE:GFP expression during the lifecycle of germ cell development.

(A) mESCs derived from Dazl−/− blastocysts display a reduction in pluripotency and germ cell markers, and do not differentiate towards the germ cell lineage as seen in wildtype and Dazl heterozygous mESC lines.

(B) Gonocytes in vivo and putative germ cell in vitro show a reduction in Oct4ΔPE:GFP -positive cells, and do not display stage specific genetic markers of germ cell development, or erasure of imprinting methylation marks at the H19 locus in males.

(C) Gonia isolated from Dazl−/− mutant gonads show a significant reduction in Oct4ΔPE:GFP -positive cells and aberrant gene expression, indicating a loss of pluripotency, self-renewal, and germ cell potential.

(D) Early stage oocytes and spermatocytes from Dazl−/− embryos display a significant loss of Oct4ΔPE:GFP -positive cells, aberrant gene expression, failure to re-establish imprinting methylation marks at the H19 locus in males, and failure progress though meiosis.

"Mammalian germ cells progress through a unique developmental program that encompasses proliferation and migration of the nascent primordial germ cell (PGC) population, reprogramming of nuclear DNA to reset imprinted gene expression, and differentiation of mature gametes. Little is known of the genes that regulate quantitative and qualitative aspects of early mammalian germ cell development both in vivo, and during differentiation of germ cells from mouse embryonic stem cells (mESCs) in vitro."

Links: primordial germ cell

Reference

Haston KM, Tung JY & Reijo Pera RA. (2009). Dazl functions in maintenance of pluripotency and genetic and epigenetic programs of differentiation in mouse primordial germ cells in vivo and in vitro. PLoS ONE , 4, e5654. PMID: 19468308 DOI.

Copyright

© 2009 Haston et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Original file name: Figure 9. Journal.pone.0005654.g009.jpg

Cite this page: Hill, M.A. (2024, April 27) Embryology Model of Dazl germ cell function.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Model_of_Dazl_germ_cell_function.jpg

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