File:Lutein cell lipid and glycogen em01.jpg

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Lutein Cell lipid and Glycogen EM

Luteln cell 25 days after mating. Glycogen accumulations (G) are packed between lipid droplets (L) of little electron opacity. This alpha glycogen consists of clumps of individual (beta) particles. Mitochondria (M) are also labeled. X27,000.

"Similar intense lead staining of the monoparticulate, beta glycogen has been correlated with the more reliable criterion of PAS staining. Glycogen accumulations which increase during the later stages of the lutein cell life-span occur as glycogen aggregates freely dispersed in the cytoplasmic matrix. At this stage, the cells have also accumulated numerous, light-appearing lipid droplets which are present during presumed "storage" phases of cellular activity." (text from original article)




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Ovarian steroid cells. I. Differentiation of the lutein cell from the granulosa follicle cell during the preovulatory stage and under the influence of exogenous gonadotrophins

J Cell Biol. 1966 Dec;31(3):501-16.

Blanchette EJ.


The granulosa follicle cell of the Graafian follicle of the rabbit ovary differentiates into a lutein cell involved in steroid synthesis. Cytological events which occur within the granulosa cell of the normally stimulated follicle prior to ovulation have been duplicated by the intrafollicular injection of exogenous gonadotrophin. The luteinization of the granulosa cells involves the accumulation of 250- to 300-A, electron-opaque, spherical granules, dispersed within the cytoplasmic matrix, which have been identified as glycogen with the PAS-staining procedure. Further development of the granulosa cell following ovulation involves an increase in cell size, a decrease in the number of RNP particles, and an accumulation of an abundant system of intracellular membranes (agranular endoplasmic reticulum). Glycogen granules first appear in the granulosa cells as the separate, monoparticulate form. After follicle rupture and the formation of agranular endoplasmic reticulum, glycogen particles are present in a rosette arrangement within membrane-bounded vacuoles. The rosette arrangement of glycogen particles is also found dispersed within the cytoplasmic matrix of the lutein cell during the later stages of the cell life-span. Injection of luteinizing hormone or human chorionic gonadotrophin into a mature follicle also produces a marked accumulation of monoparticulate glycogen in the majority of granulosa cells, within 30 min. Cytoplasmic extensions which contain the glycogen masses are noticeably free of RNP particles.

PMID 5971645

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current01:16, 14 February 2013Thumbnail for version as of 01:16, 14 February 20131,156 × 828 (149 KB)Z8600021 (talk | contribs)==Lutein Cell lipid and Glycogen EM== ==Reference== <pubmed>5971645</pubmed> {{JCB}} ---- ===Ovarian steroid cells. I. Differentiation of the lutein cell from the granulosa follicle cell during the preovulatory stage and under the influence of ex

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