File:Liver sinusoidal endothelial cell fenestrations.jpg

Liver Sinusoidal Endothelial Cell Fenestrations

Liver sinusoidal endothelial cells (LSECs) act as a filter between blood and the hepatocytes. LSECs are highly fenestrated cells; they contain transcellular pores with diameters between 50 to 200 nm. The small sizes of the fenestrae have so far prohibited any functional analysis with standard and advanced light microscopy techniques. Organised as “sieve plates”, assemblies of fenestrations in regions where LSECs are extremely thin (approx. 300 nm on average).

(B,D) dSTORM images of a fixed rat LSECs stained with Vybrant DiD. The cells were mounted in the reducing buffer OSS + MEA. (D) show enlarged view of the corresponding ROIs shown in (B). The exposure time for a single dSTORM frame was 20 ms and 15000 frames were processed to reconstruct the images shown in (B,D).

Reference

Mönkemöller V, Øie C, Hübner W, Huser T & McCourt P. (2015). Multimodal super-resolution optical microscopy visualizes the close connection between membrane and the cytoskeleton in liver sinusoidal endothelial cell fenestrations. Sci Rep , 5, 16279. PMID: 26549018 DOI.

Mönkemöller, V. et al. Multimodal super-resolution optical microscopy visualizes the close connection between membrane and the cytoskeleton in liver sinusoidal endothelial cell fenestrations. Sci. Rep. 5, 16279; doi: 10.1038/srep16279 (2015).

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Cite this page: Hill, M.A. (2024, April 27) Embryology Liver sinusoidal endothelial cell fenestrations.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Liver_sinusoidal_endothelial_cell_fenestrations.jpg

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© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G