File:Liver polyploidy 01.jpg

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Liver Polyploidy

Polyploid hepatocyte mitoses in vitro with multipolar spindles and chromosome segregation defects.

  • A - bipolar spindles were established and maintained by centrosome clustering (in about half of tetraploid mitoses)
  • B C - centrosomes oriented on 3-4 distinct poles (reflecting either true multipolar spindles or alignment of prometaphase chromosomes from binucleated hepatocytes)
  • D - octaploid hepatocytes established multipolar spindles with as many as 8 poles
  • E - about 1% of cells in anaphase or telophase were oriented with tripolar spindles
  • F - hepatocytes with 2 discrete mitotic spindles synchronized in metaphase
  • G - hepatocytes with 2 discrete mitotic spindles synchronized in anaphase
  • I - L - mitotic figures similar to those seen in cultured hepatocytes were also seen during hepatocyte proliferation in vivo

See supplementary movies for recordings of mitotic divisions.


Methods

  • cells were fixed with methanol
  • Primary antibodies for alpha-tubulin (clone DM1A, Sigma)
  • Primary antibodies were detected with species-specific secondaries conjugated to Alexafluor 488 or Alexafluor 555 (Invitrogen).
  • Nuclei were stained with 40 μg/ml Hoechst 33342 (Invitrogen).

Original File Name: Fig. 3 Nature09414-f3.2.jpg

Reference

Duncan AW, Taylor MH, Hickey RD, Hanlon Newell AE, Lenzi ML, Olson SB, Finegold MJ & Grompe M. (2010). The ploidy conveyor of mature hepatocytes as a source of genetic variation. Nature , 467, 707-10. PMID: 20861837 DOI.

Copyright

Reprinted by permission from Macmillan Publishers Ltd.

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Cite this page: Hill, M.A. (2024, April 18) Embryology Liver polyploidy 01.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Liver_polyploidy_01.jpg

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