File:Limb AER BMP expression01.jpg

From Embryology

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Limb Apical Ectodermal Ridge BMP Expression=

Removal of Bmp2, Bmp4, and Bmp7 from the AER resulted in an initial broadening and then loss of Fgf8 expression in the AER but normal Fgf10 expression.

In the forelimb, where the Msx2-cre allele instigates recombination after the AER has formed, a slight broadening of Fgf8 expression was observed in the AER (A-C).

  • E10.5 hindlimb buds containing only a single allele of Bmp7 (H; Bmp7 f/+; Bmp2f/f, Bmp4 f/f, Msx2-cre) or no functional Bmp alleles (I; Bmp7 f/f; Bmp2f/f, Bmp4 f/f, Msx2-cre), Fgf8 expression was broader throughout the AER.
  • E11.5 limb buds, Fgf8 expression was severely decreased in the central and anterior AER in embryos that contained only a single allele of Bmp7 (E and K; Bmp7 f/+; Bmp2f/f, Bmp4 f/f, Msx2-cre) or no functional Bmp alleles (F and L; Bmp7 f/f; Bmp2f/f, Bmp4 f/f, Msx2-cre).

In E10.5 hindlimb buds, Fgf10 expression was not reduced in the limb bud mesenchyme that contained only a single allele of Bmp7 or no functional Bmp alleles. The morphology of the distal limb bud was irregular in hindlimbs that contained only one functional Bmp allele (K, Q) or lacked all Bmp alleles (L, R). No differences in Fgf10 expression were observed in the forelimbs.


Links: Bone Morphogenetic Protein | Limb Development

Reference

Bmp2, Bmp4 and Bmp7 are co-required in the mouse AER for normal digit patterning but not limb outgrowth[1]

Copyright

© 2012 Choi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Figure 3. http://dx.doi.org/10.1371/journal.pone.0037826.g003


Cite this page: Hill, M.A. (2024, June 20) Embryology Limb AER BMP expression01.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Limb_AER_BMP_expression01.jpg

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© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G
  1. <pubmed>22662233</pubmed>| PLoS One.

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current08:34, 8 April 2016Thumbnail for version as of 08:34, 8 April 20161,200 × 721 (141 KB)Z8600021 (talk | contribs)Figure 3. Removal of Bmp2, Bmp4, and Bmp7 from the AER resulted in an initial broadening and then loss of Fgf8 expression in the AER but normal Fgf10 expression. In the forelimb, where the Msx2-cre allele instigates recombination after the AER has form...

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