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==Leydig Cell EM==
Opossum testicular interstitial cells showing extensive smooth endoplasmic reticulum involved in testosterone synthesis.
A low-power electron micrograph showing several interstitial cells, one of which (above) contains cytoplasm of considerably greater density than the others. The most striking feature of the interstitial cells is the abundant agranular endoplasmic reticulmn (agr), which fills their cytoplasm and is in the form of a network of interconnected tubules. At the periphery of thc cells is an ectoplasmic zone (ect), which is relatively free of endoplasmic reticulum. The cytoplasm also contains mitochondria (m), in which large, homogeneous granules (g) sometimes occur (see Fig. 10). Various extensions of the Golgi zone (G) arc seen around the nucleus. Small, dark bodies (b) of unknown nature are also found in the cytoplasm. The denser cell contains an oblique section through a bundle (bun) of minute tubules, but it is difficult to make out detail because of the great density of the cytoplasm.
A low-power electron micrograph showing several interstitial cells, one of which (above) contains cytoplasm of considerably greater density than the others. The most striking feature of the interstitial cells is the abundant agranular endoplasmic reticulmn (agr), which fills their cytoplasm and is in the form of a network of interconnected tubules. At the periphery of thc cells is an ectoplasmic zone (ect), which is relatively free of endoplasmic reticulum. The cytoplasm also contains mitochondria (m), in which large, homogeneous granules (g) sometimes occur (see Fig. 10). Various extensions of the Golgi zone (G) arc seen around the nucleus. Small, dark bodies (b) of unknown nature are also found in the cytoplasm. The denser cell contains an oblique section through a bundle (bun) of minute tubules, but it is difficult to make out detail because of the great density of the cytoplasm.
The edge of a seminiferous tubule (sere) is seen at lower right, and is flanked by one of the cells (lain) which contribute to the lamina propria of the tubule. X 6,200.
The edge of a seminiferous tubule (sere) is seen at lower right, and is flanked by one of the cells (lain) which contribute to the lamina propria of the tubule. X 6,200.


{{Leydig cell EM links}}
===Reference===


J Biophys Biochem Cytol. 1961 Mar;9:653-70.
<pubmed>13693345</pubmed>
The normal fine structure of opossum testicular interstitial cells.
CHRISTENSEN AK, FAWCETT DW.
Abstract
The interstitial tissue of the opossum testis includes interstitial or Leydig cells, macrophages, and small cells which morphologically resemble mesenchymal cells. The latter are thought to give rise to mature interstitial cells. The most prominent feature of the interstitial cell cytoplasm is an exceedingly abundant agranular endoplasmic reticulum. This reticulum is generally in the form of a meshwork of interconnected tubules about 300 to 450 A in diameter, but occasionally it assumes the form of flattened, fenestrated cisternae resembling those of pancreatic acinar cells, except for the lack of ribonucleoprotein particles on the surface of the membranes. The interstitial cells vary considerably in their cytoplasmic density. The majority are quite light, but some appear extremely dense, and in addition usually have a more irregular cell surface, with numerous small pseudopodia. These differences may well reflect variations in physiological state. Cytoplasmic structures previously interpreted as "crystalloids" consist of long bundles of minute parallel tubules, each about 180 A in diameter, which seem to be local differentiations of the endoplasmic reticulum. The mitochondria are rod-shaped, and contain a moderately complex internal membrane structure, and also occasional large inclusions that are spherical and homogeneous. The prominent juxtanuclear Golgi complex contains closely packed flattened sacs and small vesicles. The results of the present study, coupled with biochemical evidence from other laboratories, make it seem highly probable that the agranular endoplasmic reticulum is involved in the synthesis of the steroid hormones produced by the interstitial cell. This finding therefore constitutes one of the first functions of the agranular reticulum for which there is good morphological and biochemical evidence.


PMID 13693345
{{JCB}}


{{JCB}}
Original figure 3 rotated, relabelled and adjusted in size and contrast.

Revision as of 17:20, 7 August 2014

Leydig Cell EM

Opossum testicular interstitial cells showing extensive smooth endoplasmic reticulum involved in testosterone synthesis.

A low-power electron micrograph showing several interstitial cells, one of which (above) contains cytoplasm of considerably greater density than the others. The most striking feature of the interstitial cells is the abundant agranular endoplasmic reticulmn (agr), which fills their cytoplasm and is in the form of a network of interconnected tubules. At the periphery of thc cells is an ectoplasmic zone (ect), which is relatively free of endoplasmic reticulum. The cytoplasm also contains mitochondria (m), in which large, homogeneous granules (g) sometimes occur (see Fig. 10). Various extensions of the Golgi zone (G) arc seen around the nucleus. Small, dark bodies (b) of unknown nature are also found in the cytoplasm. The denser cell contains an oblique section through a bundle (bun) of minute tubules, but it is difficult to make out detail because of the great density of the cytoplasm. The edge of a seminiferous tubule (sere) is seen at lower right, and is flanked by one of the cells (lain) which contribute to the lamina propria of the tubule. X 6,200.

Leydig Cell Links: Low power EM | High power EM | Testis Development


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Historic Embryology - Genital 
General: 1901 Urinogenital Tract | 1902 The Uro-Genital System | 1904 Ovary and Testis | 1912 Urinogenital Organ Development | 1914 External Genitalia | 1921 Urogenital Development | 1921 External Genital | 1942 Sex Cords | 1953 Germ Cells | Historic Embryology Papers | Historic Disclaimer
Female: 1904 Ovary and Testis | 1904 Hymen | 1912 Urinogenital Organ Development | 1914 External Genitalia | 1914 Female | 1921 External Genital | 1927 Female Foetus 15 cm | 1927 Vagina | 1932 Postnatal Ovary
Male: 1887-88 Testis | 1904 Ovary and Testis | 1904 Leydig Cells | 1906 Testis vascular | 1909 Prostate | 1912 Prostate | 1914 External Genitalia | 1915 Cowper’s and Bartholin’s Glands | 1920 Wolffian tubules | 1935 Prepuce | 1935 Wolffian Duct | 1942 Sex Cords | 1943 Testes Descent | Historic Embryology Papers | Historic Disclaimer

Reference

<pubmed>13693345</pubmed>

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current17:13, 7 August 2014Thumbnail for version as of 17:13, 7 August 20141,359 × 957 (341 KB)Z8600021 (talk | contribs)J Biophys Biochem Cytol. 1961 Mar;9:653-70. The normal fine structure of opossum testicular interstitial cells. CHRISTENSEN AK, FAWCETT DW. Abstract The interstitial tissue of the opossum testis includes interstitial or Leydig cells, macrophages, and s...

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