File:Human trophoblasts stained with different markers.jpg

Expression of IMUP-2 in HTR-8/SVneo trophoblast.

(A) Expression of IMUP-2 in HTR-8/SVneo trophoblast using RT-PCR. (B) Densitometric analysis of IMUP-2 mRNA expression relative to 28s rRNA mRNA expression. Data are expressed as means±standard error (SE). Statistical analysis was carried out by Student’s t-tests (*P<0.05). (C) Expression of IMUP-2 in HTR-8/SVneo trophoblast using Western blot analysis. GAPDH was used as a internal control. (D) Immunofluorescence for IMUP-2 in HTR-8/SVneo trophoblast cells after transfection of pcDNA3/IMUP-2 and siRNA/IMUP-2 (×200). Arrow means positive signals for IMUP-2 in HTR-8/SVneo trophoblast. (Mock: lipofectamin transfection; IMUP-2: pcDNA3/ IMUP-2 transfection; siIMUP-2: siRNA/IMUP-2 transfection).

Reference

<pubmed>25949126</pubmed>

Jung R, Choi JH, Lee HJ, Kim JK, Kim GJ. Effect of Immortalization-Upregulated Protein-2 (IMUP-2) on Cell Death of Trophoblast. Development & Reproduction. 2013;17(2):99-109. doi:10.12717/DR.2013.17.2.099.

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