File:Human spermatid electron micrograph.jpg

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Human Spermatid Electron Micrograph

Electron micrographs of human spermatids following post-embedding labelling with anti-SPANXa/d antibodies.

  • A - cap-phase spermatid staining was not observed associated with the nuclear envelope (ne) underlying the developing acrosome (a). However, gold particles (5 nm) were present on membrane whorls lying within a nuclear vacuole (NV) that opened into the subacrosomal space.
  • B - elongated spermatid gold particles were associated with both the nucleoplasmic and the cytoplasmic faces of the nuclear envelope (ne) posterior to the acrosome (a) and adjacent to the manchette microtubules (M, white arrows). Staining of the nuclear envelope was not observed underlying the developing acrosome (a, bars mark posterior aspect of acrosome) or in the region immediately posterior to the acrosomal bulbs where the nuclear envelope is closely apposed to the nucleus.

The inset shows a fold of the nuclear envelope at the posterior region of the spermatid nucleus which exhibited a concentration of gold particles indicative of SPANXa/d staining. M, manchette; N, nucleus.

A × 20,000; B × 16,000.


Human Spermatozoa EM: Image - cap-phase spermatid | Image - elongated spermatid | Image - spermatid | Image - spermatozoa | Image - normal nucleus | Image - nucleus | Image - abnormal nucleus | Spermatozoa Development | Category:Electron Micrograph


Spermatozoa Images: Spermatozoa BF | Spermatozoon BF | Spermatozoon EM | Spermatozoon EM | Historic drawing | Category:Spermatozoa | Spermatozoa Development | Testis Development

Reference

<pubmed>17012309</pubmed>| Mol Hum Reprod.


Copyright

© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

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Original file name: Figure 8. http://molehr.oxfordjournals.org/content/12/11/703/F8.expansion.html

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current16:11, 1 November 2010Thumbnail for version as of 16:11, 1 November 2010619 × 918 (206 KB)S8600021 (talk | contribs)==Human Spermatid EM== Electron micrographs of human spermatids following post-embedding labelling with anti-SPANXa/d antibodies. (A) In this cap-phase spermatid staining was not observed associated with the nuclear envelope (ne) underlying the develop

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