File:Human fetal gonad retinoid receptor expression.jpg: Difference between revisions

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Immunohistochemical localisation of retinoid receptor expression in the human fetal gonad

Second trimester human fetal

(A) testis RARα staining was detected in germ cell (GC) and peritubular myoid (PTM) nuclei. Two populations of Sertoli cells (SC; immunopositive and immunonegative) could also be detected.

(B) ovary (same developmental stage) RARα expression was detected in the nuclei and cytoplasm of germ cells in nests, and in the nuclei of pregranulosa cells (PG) interspersed between germ cells. Mesenchymal somatic cells in streams (CS) displayed variable staining.

(C) testis RARβ expression was widespread with all major cell populations displaying intense nuclear staining.

(D) ovary variable RARβ expression was detected in the germ cells with some displaying intensely stained nuclei or both nuclear and cytoplasmic staining (solid arrows) and others showing little or no staining (dashed arrows). Pregranulosa cells were immunonegative, as were somatic cells in streams, although some displayed nuclear staining for RARβ (arrowheads).

(E) testis RARβ peritubular myoid and Sertoli cell nuclei displayed intense staining, with weaker expression in detected in germ cells. A population of immunonegative IC was also detected.

(F) ovary distribution of immunostaining for RXRα was comparable to that of RARβ, with expression restricted to germ cells in nests (GCn) and absent in somatic cell streams and pregranulosa cells. The widespread nuclear localization of RA receptors in testis suggests cells of all types (including germ cells) are exposed to RA signals.

Magnification: 400× (A, B), 1000× (C–F).

Original File Name: Figure 3 Journal.pone.0020249.g003.png doi:10.1371/journal.pone.0020249.g003 (adjust size from original)

Reference

Childs AJ, Cowan G, Kinnell HL, Anderson RA, Saunders PTK (2011) Retinoic Acid Signalling and the Control of Meiotic Entry in the Human Fetal Gonad. PLoS ONE 6(6): e20249. PMID 21674038 | PLoS One

Received: November 29, 2010; Accepted: April 28, 2011; Published: June 3, 2011

Copyright: © 2011 Childs et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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current	15:41, 18 June 2011		1,004 × 1,000 (447 KB)	S8600021 (talk \| contribs)	==Immunohistochemical localisation of retinoid receptor expression in the human fetal gonad== In the second trimester human fetal testis (A) RARα staining was detected in germ cell (GC) and peritubular myoid (PTM) nuclei. Two populations of Sertoli cell

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Developmental Signals - Retinoic acid

@@ Line 1: / Line 1: @@
 ==Immunohistochemical localisation of retinoid receptor expression in the human fetal gonad==
-In the second trimester human fetal testis (A) RARα staining was detected in germ cell (GC) and peritubular myoid (PTM) nuclei. Two populations of Sertoli cells (SC; immunopositive and immunonegative) could also be detected. In the fetal ovary at the same developmental stage (B), RARα expression was detected in the nuclei and cytoplasm of germ cells in nests, and in the nuclei of pregranulosa cells (PG) interspersed between germ cells. Mesenchymal somatic cells in streams (CS) displayed variable staining. RARβ expression was widespread in the fetal testis (C) with all major cell populations displaying intense nuclear staining. In contrast, variable RARβ expression was detected in the germ cells of the fetal ovary (D); with some displaying intensely stained nuclei or both nuclear and cytoplasmic staining (solid arrows) and others showing little or no staining (dashed arrows). Pregranulosa cells were immunonegative, as were somatic cells in streams, although some displayed nuclear staining for RARβ (arrowheads). Peritubular myoid and Sertoli cell nuclei in the testes displayed intense staining for RARβ (E), with weaker expression in detected in germ cells. A population of immunonegative IC was also detected. The distribution of immunostaining for RXRα in the fetal ovary (F) was comparable to that of RARβ, with expression restricted to germ cells in nests (GCn) and absent in somatic cell streams and pregranulosa cells. The widespread nuclear localization of RA receptors in testis suggests cells of all types (including germ cells) are exposed to RA signals. Magnification: 400× (A, B), 1000× (C–F).
+Second trimester human fetal
+(A) testis  RARα staining was detected in germ cell (GC) and peritubular myoid (PTM) nuclei. Two populations of Sertoli cells (SC; immunopositive and immunonegative) could also be detected.
+(B) ovary (same developmental stage) RARα expression was detected in the nuclei and cytoplasm of germ cells in nests, and in the nuclei of pregranulosa cells (PG) interspersed between germ cells. Mesenchymal somatic cells in streams (CS) displayed variable staining.
+(C) testis RARβ expression was widespread with all major cell populations displaying intense nuclear staining.
+(D) ovary variable RARβ expression was detected in the germ cells with some displaying intensely stained nuclei or both nuclear and cytoplasmic staining (solid arrows) and others showing little or no staining (dashed arrows). Pregranulosa cells were immunonegative, as were somatic cells in streams, although some displayed nuclear staining for RARβ (arrowheads).
+(E) testis RARβ peritubular myoid and Sertoli cell nuclei displayed intense staining, with weaker expression in detected in germ cells. A population of immunonegative IC was also detected.
+(F) ovary distribution of immunostaining for RXRα was comparable to that of RARβ, with expression restricted to germ cells in nests (GCn) and absent in somatic cell streams and pregranulosa cells. The widespread nuclear localization of RA receptors in testis suggests cells of all types (including germ cells) are exposed to RA signals.
+Magnification: 400× (A, B), 1000× (C–F).
@@ Line 13: / Line 27: @@
 Copyright: © 2011 Childs et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
+[[Category:Human]] [[Category:Fetal]] [[Category:Gonad]] [[Category:Testis]] [[Category:Ovary]] [[Category:Molecular]] [[Category:Second Trimester]]