File:Human embryonic stem cell defined conditions 02.jpg

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Human Embryonic Stem (ES) Cells Differentiation

Maintenance of Human embryonic stem (ES) cells under the completely chemically defined conditions. Characterization of teratomas from H9 cells cultured on an hE-cad-Fc-coated surface.

Hematoxylin and eosin staining of paraffin sections through teratomas identified the differentiation huES cells into various tissues:

  • a - immature neuroblastic tissue with neuronal rosettes
  • b - neuroepithelium with pigment
  • c - immature sebaceous tissue
  • d - cartilage
  • e - columnar epithelium
  • f - gut-like epithelial structures
  • g - (larger image) contains neural tissue (1), cartilage (2), bone parenchyma (3), and epithelial tissue (4).


Bar indicates 100 μm. (Stain - Haematoxylin Eosin)


Original file name: Figure 2. 1471-213X-10-60-2-l.jpg http://www.biomedcentral.com/1471-213X/10/60/figure/F2 (Image cropped and resized from panel H of full figure)

Reference

<pubmed>20525219</pubmed>| BMC Dev Biol.


© 2010 Nagaoka et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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current11:54, 25 May 2011Thumbnail for version as of 11:54, 25 May 20111,091 × 411 (187 KB)S8600021 (talk | contribs)==Maintenance of Human embryonic stem (ES) cells under the completely chemically defined condition== Characterization of teratomas from H9 cells cultured on an hE-cad-Fc-coated surface. Hematoxylin and eosin staining of paraffin sections through teratoma

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