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(Human spermatozoa Image from: panel B Figure 1. TRPM8 channels are present in human sperm. A) RT-PCR showing a TRPM8 fragment amplified from human semen. Total RNA was extracted from human semen and cDNA was prepared. Specific oligonucleotides were use)
 
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Human spermatozoa
==Human Spermatozoa==


Image from: panel B
A brightfield image using phase optics of human spermatozoa.


Figure 1. TRPM8 channels are present in human sperm.
 
 
Original file name: Figure 1. TRPM8 channels are present in human sperm. (Image from: panel B)


A) RT-PCR showing a TRPM8 fragment amplified from human semen. Total RNA was extracted from human semen and cDNA was prepared. Specific oligonucleotides were used for TRPM8. Identity of the expected size (in white numbers) fragment was confirmed by DNA sequencing. B) Immunocytochemistry showing the presence of TRPM8 in heads and flagella of human sperm. Left panels show the confocal fluorescent images with pseudocolor and right panels show their corresponding phase contrast images. C) Western blot experiments showing TRPM8 reactivity (~130 kDa) in sperm lysates (anti TRPM8 from Gene Tex). n≥3.
A) RT-PCR showing a TRPM8 fragment amplified from human semen. Total RNA was extracted from human semen and cDNA was prepared. Specific oligonucleotides were used for TRPM8. Identity of the expected size (in white numbers) fragment was confirmed by DNA sequencing. B) Immunocytochemistry showing the presence of TRPM8 in heads and flagella of human sperm. Left panels show the confocal fluorescent images with pseudocolor and right panels show their corresponding phase contrast images. C) Western blot experiments showing TRPM8 reactivity (~130 kDa) in sperm lysates (anti TRPM8 from Gene Tex). n≥3.
==Reference==
<pubmed>19582168</pubmed>| [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0006095]




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Received: March 31, 2009; Accepted: May 30, 2009; Published: June 30, 2009
Received: March 31, 2009; Accepted: May 30, 2009; Published: June 30, 2009


Copyright: © 2009 De Blas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Copyright: © 2009 De Blas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




[[Category:Spermatozoa]]
[[Category:Human]] [[Category:Spermatozoa]]

Revision as of 14:14, 8 May 2011

Human Spermatozoa

A brightfield image using phase optics of human spermatozoa.


Original file name: Figure 1. TRPM8 channels are present in human sperm. (Image from: panel B)

A) RT-PCR showing a TRPM8 fragment amplified from human semen. Total RNA was extracted from human semen and cDNA was prepared. Specific oligonucleotides were used for TRPM8. Identity of the expected size (in white numbers) fragment was confirmed by DNA sequencing. B) Immunocytochemistry showing the presence of TRPM8 in heads and flagella of human sperm. Left panels show the confocal fluorescent images with pseudocolor and right panels show their corresponding phase contrast images. C) Western blot experiments showing TRPM8 reactivity (~130 kDa) in sperm lysates (anti TRPM8 from Gene Tex). n≥3.

Reference

<pubmed>19582168</pubmed>| [1]


Citation: De Blas GA, Darszon A, Ocampo AY, Serrano CJ, Castellano LE, et al. (2009) TRPM8, a Versatile Channel in Human Sperm. PLoS ONE 4(6): e6095. doi:10.1371/journal.pone.0006095

http://www.ncbi.nlm.nih.gov/pubmed/19582168

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0006095

Editor: Wen-Liang Zhou, Sun Yat-Sen University, China

Received: March 31, 2009; Accepted: May 30, 2009; Published: June 30, 2009


Copyright: © 2009 De Blas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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