File:Human- vacuolated spermatozoa.jpg

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Human Spermatozoa Vacuolated Morphology

B: Spermatozoa with large nuclear vacuoles observed at high magnification (≥8400×).

Spermatozoa were analyzed at greater than or equal to 8400x magnification by inverted microscope equipped with DIC/Nomarski differential interference contrast optics. At least 200 motile spermatozoa per semen sample were evaluated and percentages of normal spermatozoa and spermatozoa with large nuclear vacuoles (LNV/one or more vacuoles occupying>50% of the sperm nuclear area) were determined. A spermatozoon was classified as morphologically normal when it exhibited a normal nucleus (smooth, symmetric and oval nucleus, width 3.28+/-0.20 microm, length 4.75+/-0.20 microm/absence of vacuoles occupying>4% of nuclear area) as well as acrosome, post-acrosomal lamina, neck and tail, besides not presenting cytoplasm around the head. One examiner, blinded to subject identity, performed the entire study.

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Reference

<pubmed>20529256</pubmed>| PMC2889997 | Reprod Biol Endocrinol.

© 2010 Oliveira et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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current12:19, 22 September 2010Thumbnail for version as of 12:19, 22 September 20101,000 × 801 (77 KB)S8600021 (talk | contribs)==Human Spermatozoa Vacuolated Morphology== B: Spermatozoa with large nuclear vacuoles observed at high magnification (≥8400×). Spermatozoa were analyzed at greater than or equal to 8400x magnification by inverted microscope equipped with DIC/Nomarsk

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