File:Blastocyst pluripotency markers 01.jpg: Difference between revisions

From Embryology
mNo edit summary
mNo edit summary
 
(One intermediate revision by the same user not shown)
Line 4: Line 4:


* '''a''' - red alkaline phosphatase (AP) activity.
* '''a''' - red alkaline phosphatase (AP) activity.
* '''b''' - red Oct-4 expression.
* '''b''' - green Oct-4 expression.
* '''c''' - green TROMA-1 expression (cytokeratin 8, Endo A; cytokeratin-like filaments present in trophectoderm and endodermal cells)
* '''c''' - green TROMA-1 expression (cytokeratin 8, Endo A; cytokeratin-like filaments present in trophectoderm and endodermal cells)


Line 10: Line 10:




:'''Links:''' [[Blastocyst]] | [[Week 1]] | [[Stem Cells]] [[Trophoblast]]
:'''Links:''' [[Blastocyst]] | [[Week 1]] | [[Stem Cells]] | [[Trophoblast]]


===Reference===
===Reference===

Latest revision as of 12:03, 5 May 2013

Localization of Pluripotency Markers

Hatched mouse blastocysts alkaline phosphatase activity and Oct-4 are markers for pluripotency, while TROMA-1 is a marker for trophectoderm specification.

  • a - red alkaline phosphatase (AP) activity.
  • b - green Oct-4 expression.
  • c - green TROMA-1 expression (cytokeratin 8, Endo A; cytokeratin-like filaments present in trophectoderm and endodermal cells)

blue - DAPI was used for nuclear DNA counterstaining. Scale = 30 μm.


Links: Blastocyst | Week 1 | Stem Cells | Trophoblast

Reference

<pubmed>16681851</pubmed>| PMC1479373 | J Transl Med.

Copyright

© 2006 Tanaka et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Figure 5. 1479-5876-4-20-5-l.jpg

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current11:25, 5 May 2013Thumbnail for version as of 11:25, 5 May 20131,197 × 371 (54 KB)Z8600021 (talk | contribs)==Localization of pluripotency markers== Alkaline phosphatase (AP) activity (left), Oct-4 (center), and TROMA-1 (right) expression as measured in expanded and hatched blastocysts (top row, a-c) by immunofluorescent analysis. * a - red alkaline phosph...

There are no pages that use this file.