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(==Localization of pluripotency markers== Alkaline phosphatase (AP) activity (left), Oct-4 (center), and TROMA-1 (right) expression as measured in expanded and hatched blastocysts (top row, a-c) by immunofluorescent analysis. * a - red alkaline phosph...)
 
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Alkaline phosphatase (AP) activity (left), Oct-4 (center), and TROMA-1 (right) expression as measured in expanded and hatched blastocysts (top row, a-c) by immunofluorescent analysis.  
Alkaline phosphatase (AP) activity (left), Oct-4 (center), and TROMA-1 (right) expression as measured in expanded and hatched blastocysts (top row, a-c) by immunofluorescent analysis.  
* a - red alkaline phosphatase (AP) activity.
* '''a''' - red alkaline phosphatase (AP) activity.
* b - red Oct-4 expression.
* '''b''' - red Oct-4 expression.
* c - green TROMA-1 expression  
* '''c''' - green TROMA-1 expression  


blue - DAPI was used for nuclear DNA counterstaining.  Scale = 30 μm.
blue - DAPI was used for nuclear DNA counterstaining.  Scale = 30 μm.
:'''Links:''' [[Blastocyst]] | [[Week 1]] | [[Stem Cells]]


===Reference===
===Reference===


Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: applications and preliminary results in a murine model.
 
Tanaka N, Takeuchi T, Neri QV, Sills ES, Palermo GD.
<pubmed>16681851</pubmed>
J Transl Med. 2006 May 8;4:20.
PMID: 16681851


http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1479373/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1479373/

Revision as of 11:30, 5 May 2013

Localization of pluripotency markers

Alkaline phosphatase (AP) activity (left), Oct-4 (center), and TROMA-1 (right) expression as measured in expanded and hatched blastocysts (top row, a-c) by immunofluorescent analysis.

  • a - red alkaline phosphatase (AP) activity.
  • b - red Oct-4 expression.
  • c - green TROMA-1 expression

blue - DAPI was used for nuclear DNA counterstaining. Scale = 30 μm.


Links: Blastocyst | Week 1 | Stem Cells

Reference

<pubmed>16681851</pubmed>

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1479373/

http://www.translational-medicine.com/content/4/1/20

Copyright

© 2006 Tanaka et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Figure 5. 1479-5876-4-20-5-l.jpg

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