File:Bacteria - gram-stained vaginal smear 01.jpg

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Gram-Stained Vaginal Smear

Gram-stained vaginal smear showing mainly Lactobacillus crispatus cell types.

  • plump quite homogeneous lactobacilli
  • grade I microflora (suggested new grade 1a)
  • microscopic image (100×)
Smear Image Links: L. crispatus | L. crispatus | non-L. crispatus with thin lactobacilli | non-L. crispatus with thin lactobacilli | mixture non-L. crispatus with L. crispatus | mixture non-L. crispatus with L. crispatus | irregular-shaped Gram positive rod | irregular-shaped Gram positive rod | mixture Lactobacillus and bacterial vaginosis-associated | mixture Lactobacillus and bacterial vaginosis-associated | bacterial vaginosis | bacterial vaginosis
Links: Menstrual Cycle - Histology | Histology - Gram Stain | Bacterial Vaginosis | CDC (USA) Fact Sheet - Bacterial Vaginosis

Microflora Grade

  • Grade I - when only Lactobacillus cell types (large Gram positive rods) were present.
  • Grade II - (intermediate) when both Lactobacillus and Gardnerella or Bacteroides-Prevotella cell types were present.
  • Grade III - (bacterial vaginosis) when Lactobacillus cell types were absent and only Gardnerella, Bacteroides-Prevotella or Mobiluncus cell types were present.
  • Grade IV - when Gram positive cocci were predominantly present.


<pubmed>16225680</pubmed>| PMC1266370 | BMC Microbiol.


© 2005 Verhelst et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Original file name: Figure 1. (Panel A from full figure, size and contrast adjusted)

Cite this page: Hill, M.A. (2024, February 24) Embryology Bacteria - gram-stained vaginal smear 01.jpg. Retrieved from

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© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G

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current11:25, 19 November 2011Thumbnail for version as of 11:25, 19 November 20111,000 × 749 (185 KB)S8600021 (talk | contribs)==Gram-Stained Vaginal Smear== Gram-stained vaginal smears illustrating the different categories of vaginal microflora described:. a, b: grade Ia, i.e. mainly Lactobacillus crispatus cell types, plump quite homogeneous lactobacilli. c, d: grade Ib, i.e.

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