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Adhesion molecules expression and influence in hematopoietic and endothelial differentiation
A) Knockdown of junction molecules ZO-1, Cx43 and E-Cad in murine ESC results in increased commitment to differentiate along the endothelial lineage with concurrent loss of commitment to hematopoietic cells. These fate decisions occur after the emergence of Flk-1 precursors in EB. Knockdown of ZO-2 in mouse ESC reduces hematopoietic commitment without influencing endothelial differentiation.
B) Hematopoietic stages of differentiation can be identified using intracellular and extracellular markers. ESC undergoing mesodermal commitment express the transcription factor Bry. As mesodermal cells become committed to early hematopoietic and endothelial progenitors, the cell surface receptor, Flk-1, is expressed. Scl is a transcription factor expressed as cells commit specifically to the hematopoietic lineage. Expression of E-cad, Cx43, ZO-1 and ZO-2 varies during early stages of hematopoietic and endothelial differentiation of ESC.
Figure 6. Journal.pone.0023810.g006.png doi:10.1371/journal.pone.0023810.g006
<pubmed>21789202</pubmed>| PloS ONE
© 2011 Stankovich et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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|22:11, 7 September 2011
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|==Adhesion molecules expression and influence in hematopoietic and endothelial differentiation== A) Knockdown of junction molecules ZO-1, Cx43 and E-Cad in murine ESC results in increased commitment to differentiate along the endothelial lineage with con