Difference between revisions of "ANAT2341 Lab 2"

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== 1. QUIZ ==
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PRACTICAL CLASS PROGRAM:
  
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* Weekly Quiz + revision (15 minutes)
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* Practical class activities: (45 minutes)
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* Guest Lecture by [https://wch.med.unsw.edu.au/people/associate-professor-robert-gilchrist Professor Robert Gilchrist] (45 minutes)
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* Practical Class Revision (15 minutes)
  
== 2. Guest Lecturer - Dr Fabien Delerue ==
 
{|
 
| width=185px|[[File:Fabien deleRue profile photo.jpg|180px]]
 
  
Dr Fabien Delerue
 
| '''Manipulating the mouse embryo: from ES Cells to genome editing'''
 
<br>
 
Dr Delerue is currently in the Transgenic Animal Unit (TAU) his career spans over twenty years practice in animal handling, surgery, and generation of animal models of diseases. His research focuses on the production and characterization of new transgenic mouse models of human genetic disorders, using cutting-edge genome engineering techniques such as CRISPR/Cas9.
 
  
Links: [https://research.unsw.edu.au/people/dr-fabien-delerue UNSW Research Gateway]
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PRACTICAL CLASS ACTIVITIES:
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'''Lecture slides:''' [[File:Delerue.pdf]]
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# Virtual human embryo dissections and histology
===References===
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# Embryo models and specimens of human developmental abnormalities (optional)
  
Delerue F & Ittner LM. Genome Editing in Mice Using CRISPR/Cas9: Achievements and Prospects
 
Cloning and Transgenesis 2015, 4:135 doi:10.4172/2168-9849.1000135 [http://www.omicsgroup.org/journals/genome-editing-in-mice-using-crisprcas9-achievements-and-prospects-2168-9849-1000135.php?aid=50992]
 
  
:"Animal models are a powerful tool to understand the mechanisms underlying physiological and pathological processes in vivo. To date, mice remain the species most commonly used for genetic manipulation. The recent development of engineered endonucleases such as Zinc Finger Nucleases (ZFN), Transcription activator-like effector nucleases  (TALEN), and the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) offered easy, flexible, and fast alternative to ES-Cell based gene targeting. Thanks to multiple advantages, the CRISPR system superseded its predecessors and became a popular method for genome editing. Here, we review the latest techniques to apply CRISPR editing to the mouse genome, and emphasize on the current methods used in transgenic laboratories and subsequent achievements in mice."
 
  
Ke YD, van Hummel A, Stevens CH, Gladbach A, Ippati S, Bi M, Lee WS, Krüger S, van der Hoven J, Volkerling A, Bongers A, Halliday G, Haass NK, Kiernan M, Delerue F, Ittner LM.
Short-term suppression of A315T mutant human TDP-43 expression improves functional deficits in a novel inducible transgenic mouse model of FTLD-TDP and ALS. Acta Neuropathologica 2015 Nov;130(5):661-78 PubMed 26437864 [https://dx.doi.org/10.1007/s00401-015-1486-0]
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LEARNING OBJECTIVES:
  
Ittner A, Chua SW, Bertz J, Volkerling A, van der Hoven J, Gladbach A, Przybyla M, Bi M, van Hummel A, Stevens CH, Ippati S, Suh LS, Macmillan A, Sutherland G, Kril JJ, Silva APG, Mackay J, Poljak A, Delerue F,  Ke YD, Ittner LM. Site-specific phosphorylation of tau inhibits amyloid-β toxicity in Alzheimer’s mice
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* Understanding of reproductive technologies
Science 2016 Nov;354 (6314):904-908 doi: 10.1126/science.aah6205 [http://science.sciencemag.org/content/354/6314/904.full]
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* Understanding of events during early ectoderm and mesoderm development
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* Understanding of the process of neurulation and neural crest formation
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* Understanding of the adult components derived from ectoderm and mesoderm
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* Understanding the process of body cavity formation
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* Brief understanding of early heart formation
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* Brief understanding of abnormalities associated with early ectoderm and mesoderm development
  
===Recommended Literature===
 
{|
 
| [[File:Manipulating_the_Mouse_Embryo.jpg|200px]]
 
| | [[File:Reproductive Engineering Techniques.jpg|200px]]
 
|-
 
| [http://cshlpress.com/default.tpl?cart=147944968860031853&action=full&--eqskudatarq=982 Manipulating the Mouse Embryo: A Laboratory Manual (Fourth Edition)] By Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, & Andras Nagy.
 
| [http://card.medic.kumamoto-u.ac.jp/card/english/sigen/manual/onlinemanual.html Reproductive Engineering techniques in mice: Technical manual] Prof Naomi Nakagata, Center for Animal Resources and Development, Kumamoto University, Japan
 
|}
 
  
===External Links===
 
{{External Links}}
 
* NCBI Search - [http://www.ncbi.nlm.nih.gov/gquery/?term=CrispR NCBI databases - CRISPR] |  [http://www.ncbi.nlm.nih.gov/pubmed/?term=CrispR PubMed CRISPR] | [http://www.ncbi.nlm.nih.gov/pmc/?term=CrispR PubMed Centrap CRISPR]
 
* JoVE - [https://www.jove.com/video/55765/generation-genetically-modified-mice-through-microinjection]
 
  
<html5media height="460" width="640">https://www.youtube.com/watch?v=16g6fEup2pQ</html5media>
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PRACTICAL CLASS NOTES:  
  
<br>
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The Practical class notes are available on [https://moodle.telt.unsw.edu.au/mod/url/view.php?id=2398854 Moodle].
==3. Student Group Projects==
 
 
 
 
 
{{2017ANAT2341 footer}}
 

Latest revision as of 12:41, 26 September 2019

PRACTICAL CLASS PROGRAM:

  • Weekly Quiz + revision (15 minutes)
  • Practical class activities: (45 minutes)
  • Guest Lecture by Professor Robert Gilchrist (45 minutes)
  • Practical Class Revision (15 minutes)


PRACTICAL CLASS ACTIVITIES:

  1. Virtual human embryo dissections and histology
  2. Embryo models and specimens of human developmental abnormalities (optional)


LEARNING OBJECTIVES:

  • Understanding of reproductive technologies
  • Understanding of events during early ectoderm and mesoderm development
  • Understanding of the process of neurulation and neural crest formation
  • Understanding of the adult components derived from ectoderm and mesoderm
  • Understanding the process of body cavity formation
  • Brief understanding of early heart formation
  • Brief understanding of abnormalities associated with early ectoderm and mesoderm development


PRACTICAL CLASS NOTES:

The Practical class notes are available on Moodle.