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- 09:19, 23 February 2011 S8600021 talk contribs uploaded File:Ovarian follicle growth in vitro.jpg (==A comparison of follicular growth in a 2-D vs 3-D culture system== ===2-D culture system=== With 2-D growth granulosa cell migration away from the oocyte is evident with time in culture, leaving the oocyte vulnerable for premature extrusion. ===3-D )