File:Human spermatozoa acrosomal protein SP-10.jpg

Human Spermatozoa Acrosomal Protein SP-10

Immunofluorescent/differential interference contrast (DIC) micrographs (A–H) and diagrammatic representations (a–h; modified from Holstein and Roosen-Runge, 1981) of enzyme-dissociated human testicular cells stained with 4′6-diamidino-2-phenylindole (DAPl) II for DNA (blue), fluorescein isothiocyanate (FITC) for the acrosomal protein SP-10 (green) and tetramethylrhodamine B isothiocyanate (TRITC) for the SPANXa/d protein (red).

(A) This early round spermatid demonstrates a small acrosomal vesicle (overlying the nucleus) stained with the acrosomal marker SP-10 (green). SPANXa/d staining is associated with the nuclear periphery.

(B) Early cap-phase spermatid showing SPANXa/d staining of the nuclear periphery posterior to the early acrosomal cap. As the acrosomal cap migrates posteriorly and the spermatid begins to elongate

(C–F), SPANXa/d staining is associated with the nuclear envelope posterior to the acrosomal cap.

In D and E, it is evident that a SPANXa/d-negative region intervenes between the equatorial bulbs on either side of the acrosome and the SPANXa/d-positive nuclear envelope. In elongated spermatids

(G) and testicular spermatozoa (H), SPANXa/d staining is seen in the posterior head and cytoplasmic droplet. Occasional nuclear vacuoles demonstrating SPANXa/d staining are observed (B and C, arrows).

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Legend

• Blue, DNA
• red, acrosomal protein SP-10
• green, SPANXa/d.
• A, acrosome
• An, annulus
• C, centriole
• Fs, flagellum
• M, mitochondria
• Mt, manchette
• N, nucleus
• Sb, spindle-shaped body

Original file name: Figure 6. http://molehr.oxfordjournals.org/content/12/11/703/F6.expansion.html

Reference

<pubmed>17012309</pubmed>| Mol Hum Reprod.

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