File:Mouse-embryo granzyme G.jpg

Early Embryo Granzyme G Expression (mouse)

Whole-mount embryo in situ hybridization (ISH) using a FITC-conjugated granzyme G oligonucleotide probe at different stages of normal mouse development.

(A to C) One-cell stage mouse embryos.

(D to F) Two-cell stage mouse embryos.

(G to I) Four-cell stage mouse embryos.

(J to K) Eight-cell stage mouse embryos.

• The left panels (A, D, G, and J) show embryos photographed under phase contrast imaging.
• The middle panels (B, E, H, and K) show embryos stained with Hoechst 33342 for DNA localization (blue) under fluorescence microscope observation.
• The right panels (C, F, I, and L) show embryos hybridized with a granzyme G oligonucleotide probe (green) under confocal microscopic observation.

Scale bar: 30 μm.

Reference

<pubmed>20704734</pubmed>| BMC Dev Biol.

Tsai et al. BMC Developmental Biology 2010 10:88 doi:10.1186/1471-213X-10-88

© 2010 Tsai et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.