File:Mouse CT E10.5 head.jpg

Mouse Embryo Computed Tomography

Identically scaled renderings representing 3D μCT scans of embryos (E10.5 head according to paper methods) subjected to different fixation and scanning procedures.

A, 4% formaldehyde and secondary Bouin's fixation.

B, 4% formaldehyde + 1% glutaraldehyde with Iothalamate meglumine used as a contrast agent.

C, 4% formaldehyde + 5% glutaraldehyde plus contrast agent.

All embryos are shown in right 3/4 inferolateral view.

"Typical μCT renderings are represented by the embryos depicted in Figure 2A-C. The combination of aldehyde fixation and Cysto Conray® yields images (Figure 2B-C) with a surface quality comparable to embryos fixed with Bouin's solution (Figure 2A). Bouin's-fixed embryos appear have a rougher surface texture compared to those fixed with either glutaraldehyde mixture (Figure 2B-C). The identically scaled images of Bouin's fixed embryos appeared generally smaller compared to those fixed with glutaraldehyde mixtures, but a comparison of μCT images only cannot indicate the relative roles of fixation-induced tissue shrinkage or swelling."

Original File name: 1471-213X-10-18-2-l.jpg http://www.biomedcentral.com/1471-213X/10/18/figure/F2

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