Talk:Book - Manual of Experimental Embryology

TABLE OF CONTENTS

XOS

Lot or iLUTrraATio:^* ^

TnCTBOOU or ExPEkJHEJJTAL Ej£b*wioct ivi

PART L EQUIPMENT AND INSTRUMENTS A. Oftical Outtit 3

B GuoslumoMEKT* 3

C. ilETAL iKSnUlOafTB S

D Disiirs FOR Oferatiovs akd tor Raisiko ot Oterated Axuuls io

E. STERItttATlOX or iKjnUilEXTS II

BatlOCRAFHY (A-E) IS

PART IL EXPERIMENTS ON AilPHIBIAN EilBR\OS

A. Lr.T 3 fO llATZIUAt 15

I Amphibom Commonly U«d for ExpcnmentjJ Woii &nd Tbdr Breed IngHibitJ 15

Blblttsnphy 19

a Suge Sena Rita of Devdopmest, and Other Data ao

BlUiognphy 24

5 Culture Media for Operated Embf>TM *5

Bibliogiapby a^

4 Rearittg and Feeding of Larvae 37

Bibltograpbj 3p

B Exrr.tnfryrs 30

1 Some Technical Procedure* 30

a) Eipenmcntal 0 \Til»tlon by H>-pophyta Infection and Artificial In

scminatloa In i^aaro 30

3 ) Expenmcntal CMiIatioa and Artificial Inscmisation m Undtla 35

BibEography (0-6) 37

c) Tbe Remcn.’al of Egg Membrane* 37

BIbbograpby 40

d) Narcorii 40

BibEography 41

t) Standard Equipment for Operations on Amphibian Embryo* 41

f) Some General Rula for Operation* 42

3 Tbe Pro>7wctrtT Significance of Embtj-onic Areas (\ltal Staining) 43

0) A De*criptk>Q of Gastrulatlon In Vrodtia 43

l>) rreparatioa of Dyed Agar

XI

FU

r) Vlul-stamlnx Eipenmects on tbe Early Gutxola of Vndeta (£zperi> mentJ 1,-4) 57

<0 Mtal Staining of Areai of tbe Nnmili of £7rftWa (Eiperimtnti 5-7) jg t) MtalStaimngof Lateral-Line Placodes (Experiments) 60

BibliogTaphy (o-e) 63

3 Some Anal>ilcal Expenmenti in Pregastnilation Stages 63

a) Artificial Partbenogenesis in tbe Frog b> Priding (Eipcranent 9) 63

Blbliograpby 66

b) Alteration of the Clea\*age Plane by Presanre (Experunent 10) 66

BiblK)grapb> 69

c) Tbe Prodacbon of Twin Embryos and of Duplications In Undda by

Constriction (Experiment 1 1) 69

Bibliography 74

d) Scbnltze 1 Experiment Production of Duplications by Inveraion (Ex

penment la) 75

Bibliography 76

e) Ccntrif aging (Experiment 13) 77

Bibbography 78

4 TmnsplantatiOQ Experiments To Demonstrate 'SeU-dificmtiation 79

a) Introductory Remarks Terminology 79

ft) Tramplantation of BalAncen in Uredeia (Experiment 14) Si

Bibliography 86

e) Transplantation of ForeUfflb Pranordla (Expenment 15) 86

d) Limb TnnsplantatiOQs ComUited with Irr- aj a k p of Axes (Eiperi

ment 16) 91

Bibbography (e-rf) 9*

t) Tramplantation of Gm PnmordiB (Experiment 17) 91

Blbbc^phy 94

5 Regulative Properties of Organ Primordla (hlorpbogenetk Fields) W

a) Introductory Remarks Tennlnology 94

b) Extirpation Exp erim ents on the Limb Field (Experiments 18-ai) 95

BibliogiBpby 97

c) Formation of T«o Ifearts (Expenment ss) 97

d) Partial RenKTvaJ of tbe Prospective H«irt Region (Experiment sj) 100

e) RciWTvnI of the Entire Prospective Heart Region (Experiment m)

Bibliography (c-e) lor

f) Extirpatlonof Partsof tbeE>e4onDliighlatenaIiatbeNeurulaStage (Experiments 15-^9)

Bibi»ograpb> loj

g) Partial and Total Extirpation of thcOptic Veslde (Experiments 30-31) 104

Bibbography ro5

6 Embryonic Induction

a) Introductory Remarks Tcrmlooiogy

b) Faflare of Lens Fonnatjoo afterExtlrpatioaof theEyePrimordJiim In

tbe hfeduIUiy Plate (Experiment 31)

c) Failure of Lens Formation after Extirpation of tbe Optic Verido In Early TaQ Bod Stages (Expenment 33)

i) LcnjlDductxinafterRenicr.-iloftheProT>ect[\TLciuEpitbcIIam(Ei

penmcnt 34)

Bltiwgmphj’ (fr-d)

t) The Orguuzer Eipenment TrtnsplinUtion of the Upper Lip of the Blutoport (Erpenmeot 35)

j) ImplinUtwQ of Inducton m tbeBlistococle (Eipenment 36) 114

Bibliocnphj {e-f)

7 pjuabHJsis (E\penment 3 )

Bibbograph\ 1*8

8 External Factor* ifl Development 118

c) Tbe Produakin of C> dopui ijmI Other AbaonnaliUea bj Treatment

nith Lithium Chlonde (Eipcnmcnt 38) 118

Bibliograph}

h) Eiogastrulatkin and Spina Bifida Produced bj Treatment *nth H>

pertonic Salt SolntwQ (Dvpcnmeiit 30) laa

Bibltogriphy 124

9 The Dc\'eJopfflent of BehiMor Patterns 124

a) The Ongm of Eariy Reflexes (Experiment 40) 124

b) Tbe De\'elopoicnt of Reflex Actlvitj In Najtolixed Einbrjos (Evpen

ment 4x) 127

Dibboffraphj (o-i) 12S

PART ni EXPERlifENTS ON THE CHICK EMBR\0 A. SlATEmt a:<d TcacncAi. Psocedotes 131

I Linn^ hlaterud locubatlon 131

Bibhograpb^ 133

a Limb-Bad Stages X33

Bibbography 133

3 Standard Equipment for Operations 00 Chici. Einbr>xi8 133

4- The LundvaQ Technique of Cartilage Staining i« (erfo 134

B ExpcaniEMB 136

1 The Pn>»pcctrN-e Significance of Embryonic \reas (Xltal ttaming Expen

menu) 136

o) Pro*pectnT Organ-forming Arena and Morphogenetic iloxcmeDls In the Chick ^2$

b) General Procedure of \1tat Staining 140

c) \ltal Slaimng of nen«en • Node (Experiment 4a) 143

d) Vital Staining of Part of the Vntenor Half o( the Primitire SlreaV (Ex

penment 43) 143

<•) V lUl Staining of a Preoodal Area (Fxpenment 44) 14 X

Bibliogtaph> (a-<) 14,

3 Cbono-alUnloic Grafts J4^

0) General Remarii J42

b) Chono allantoic Grafts of I irob Primordia (rxpenmeot 43) 145

f) Qwno-alJintoic Grafts of E\e Prfmordia (Experiment 46) 140

UiWrogTaph) (a-c) ,jo

sU

3 Intn-embryonk TnnspUnUtioas 150

a) Codom GnJu ctf Limb Primordlii (Experiment 47) 150

&) FUnk Grafts of Limb Pninonlli (E x per i m en t 48) 155

c) Flank Grafts of Ej-e Priroonba (Experiment 49) 135

^ Traaipfintation of Isenril Crest from Dart to ^Vhitc Breeds To Dan o&stnte tbe Oricin and 31 ip^tkm of Mclanopbores (Experiment 50) 157

BlWIofiraphy (o-rf) 159

PART IV REGENERATION (RECOVSTITimON)

A Ge-vzial Rcmaxks 163

Bibbognpby 164

B RroENzaATicw Dt Plakahia 165

I Living Material Culture Ifetbods 165

7 General Experimental Procedure 165

3 Reseneration after Transveoe, Longitudinal, and ObBqne Cutting (Ex periments 51-54) 167

4. Production of Tvo-headed Planariam (Do|JJdtas Anterior) (Experiment

55 ) 169

5 Production of Two-tafled Planarmna (Doplidtas Posterior) (Experiment

56) 1651

6 Expcrunental ProdacUon of BrpoUr forms (Polar Heteiomorpboses) (Ex

penment 57) 170

7 Production of DnpGdtas Cnoata (Experimat 58) 171

Bibliography (1-7) 171

C REmoatATTON or AuFHtBiAir Laxvae 173

I TaQ RegenereUcm (Elxpenment 59) 173

7 Limb Regeneration (Experiment 60) 174

3 Tbe ^Volffian Lens Regeneration in t/rorfef« (Experiment 61) X75

Bibliography (1-3) 178

PART V TlfE GRADIENT THEORY A. CtOtUAL RzStAKKS 183

B Extehmekts 185

I The SoiceptlbiGty Gradient In Planaria (KCN) (Experiment 6i) 185

a OiidalJon-Redacllon Gradients in the Chkt Embryo (Janus Green) (Ex penment 63) tW

3 Tbe Influence of the Axial Gradient on Head Kegeoeration In Planana

(Experiment 64) i *7

4 DcU>Td Posterior Sections (Experiment 65) 19®

Blbhography (t-4) rpr

APPENDIX

Arrvrmx Piam rou a Oxt Sanxm Covtsr *95

INDEX

iKOEt «7

XIV

LIST OF ILLUSTRATIONS

ACl

5

ncc(

I llftTRUUESTl rO» OraUTHWS ON EMUaTOS S IlYPOrnYSECTDiTV IX TTIE Fsoo

3 RzMONALor THE Jeu.\ Membraxtj or Ecc

4 Maw ot P»OOTtcrr.*E Areas or TImoox Eiarvos at the BccixxtNO OT Gasteuiattox (atter \ OCT AXD Pacteeis)

5 Mo%TiiEXTa or THE ilESODERii D Urodexe Gastruiatiox (atteji \oct)

6-10 DIACRAVI or GAlTRirtATlOX IX UROOEItS (aTTER \ OCT)

II-l6 \ ITAL STAIXINO ETTERntEXTS OX UrODEU GaSTRCUAE (aITER \ OCT AVD GoERTTIXR)

1 7 \ rtAi. Staivixo or PRospEcmT IIcaoStrcctures tx Urodeu: NrtRuiAr (ArrxR Carpexter)

iS \ ITAL StAixixo or THE Lateral UxE PiACoDE (atter Stoxe)

19 CoxsTiicnox or a Urooele Ego (s'Cell Stace) wmi a Haei » o Dutuotas Crloata k tue Fioo, Proocctd bt I'ntisioH or the Ecc (A rrrR Sormr)

31 Trasstiantattox or Baiaxcir rmiORDiA 33 Travstiavtatios or FoRrmri pRivoitou

33 Lntm Bvd TRAKtriAXTAnovs SMTH iKVCRRtox or Axes

34 pROorcnox or Heart Dctucations (aeier Cotexiuntr)

35 Exttipatiovs in the Eve FitU) or the Medvuary Fou> Stage

36 ExTTiPAnovB or E\z \ eRIcles in the Tail Bed Stage

37 ORCXXlzrR EXTTiniEKTS

aS PAixpions nxrrRiuTNT

29 rNocxsTituTiox (afttr Holtticttr)

30 Stacts or \\ rvo Bcm or Cmcx Eunkxos (aiter IIxuincrR)

31 or rioriTCTUt Oxo»>. roivlvc A«r.« in ini: Oiici; Ijitno (Arrrr I artetls)

33 llrxt>-P*ccT'< Stact Mom uevt* or Materials (aittr I AiTTris)

35 VfTxi-iTAtMNC Lxrriirvi-yTi ix the r»D3TTT\t Streak Sixrt (Arm rrm)

31 Olnato-xLt.x.VTCtC rMETTNC

33

38

48

49

49

50

39 61 ?a

73

83

6S

90

99

lot

JOS

• 13

»I 7

133

• 33

»jr

rj 9

14J

T44

X\

non

35 Limi Btm T*AKsrLA?rtATias IKTUE3 Day Cuicx EitBtvo 153

36 TtAiapLAXtATiaY or Alt Optic \esicix w mz Cmct EuBtvo (Amut

Gate*) 156

37 Heads or Dufcsui (yViUorw) doreiott^ola asd D liimto (atte* Htmak) 165

38 REOEXEEATTOX EXPElIUEIfTS IN PIjVKARIAXS 168

39 FoiEUlIB Recexeiatiox w Sauuiaxde* Laivae 17s

40 Tirr ^^0LmANLE^*

CRAPirsorDr R G Harrison Rrm Permissok or tiie AumoR Drak 1NCSB\S E. Scirtk etch) joa

z\i

TEXTBOOKS OF EXPERIMENT^VL EMBR’VOLOG'V

BETTAi-wrrY L.N-O'f andWooDCt* J H tgs 3 Modem tbfones of development London Oxford Unrvmity Pmj BttAarrr J 1945 Embrj‘ol')j!ie dumique Pans Masson et Cie

CmiD C. M 1941 Patterns and problems of doTlopmenL ChlciRO Um%-eTnt> of

Cluc»co Prtsi,

DAtxtj A, M 1933 Form aiwi caGsa£t> in faTl> de%dopmtnL Cajnbndge Eng land Cambridge Umi-crsit) Press.

DOirr'f B 193a Eipenracnttl analjiu of dexdopmenL Trans, New \ork \\ W Norton. 4 , Co

HiaLCt J S andncBcti C R 1934 Tbeclcmenuof eapenmental embrjology Cambndge En^nd Cambridge Um\enit> Pmi KoasorcLT E. I9a7-3i Regeneratwn and Transplantation. 3 s'oU, Berbn Bom Iraeger

>Ia>.coU) 0 1928 Das Detcrminatlonspfoblein I Ergtbn d BioL\oL3

19:9 Das DctorrmnatioQ problem II /W \d.5

1931 Das Determiaauoft'problein, in IhJ \ol.j

Moicas T II 1917 EipeninmijU embrjolo© \ew\ori Colombia UnI\Tnil) l‘re<

NfEDiiMi J 1931 Chemlai embf)'oloK> 3 \"ob Cambndge England Cambridge Lnivcrsll> Press.

194J BIr<b«nutf> and morph‘d ne«ts Cambridge England Cambndge

Unl«r«ri> Press

Soiuir \\ 1929 Die Determination der PnmjtiscnlwiciJang. Leiprig Alademi cbe \erUg' Gc«ellschifL

SriwsvN II 193S Erabrsotuc development and Induclioa Nciv Hairn "Vale Uni scr-its Pre^s

WAnnivCTON C H 1936 IIo*v aniouL develop New ^orL M W Norton 4 Co

torn Orginlem arul genes Cambmlge Engiand Cambndge Lnivcr'Itj

Pre*

Wll s I 1039 Pnnciples of desclopment Nr» \otL llenrj Holt

K\BOK\TOR^ MANUAT.S OF IIMPklMINTAI FJIIBR^ OIX)G\

\r lu AT iqu ^tud o m eipefirecntal ro- logv d ed \nn \rt 3r Micb TA ward Hroi Ire

Jisr 1 r 10)0 Ha cnclb> 1 fo' cipcri’ocnt on erX' r-anne an nal I h la dflpb a Ilbli ! 0

M It P lOJi I ~«nil cmbt7ol>-> fa maojal f tcrhnl-it.es and prwelurr') New \ t>»L New \ orL I c crsit) Ho* b t *e

x\u