File:Mouse meiosis pachytene 01.jpg

Spermatocytes activate a recombination-dependent arrest at pachynema

(A) Apoptotic Trip13mod/mod spermatocyte showing the axial element protein SYCP3 (green), TUNEL (green), mid/late pachytene stage marker H1t (blue) and γH2AX staining (red). Absence of H1t staining (blue) in the TUNEL-positive cell indicates that this is an early pachytene stage spermatocyte. (B) TUNEL staining appears as pan-nuclear labelling that overlaps all chromatin, which is observed in only a fraction of the analysed cells (asterisk shows an adjacent TUNEL-negative cell). (C) γH2AX localizes to the sex body (arrow) and unrepaired breaks (arrowheads). (D) Number of autosomal γH2AX patches (similar to arrowheads in (C)) counted in H1t-negative (early) and H1t-positive (mid/late) pachytene and diplotene cells from animals of the indicated genotypes. Means are indicated with horizontal lines. N shows the total number of cells counted per each stage and genotype. Primary data are provided in S1 Dataset. (E-P) Representative cells from the indicated genotypes stained for H1t (blue), γH2AX (red) and SYCP3 (green). In Trip13mod/mod, mid/late pachytene cells have substantially fewer γH2AX patches than early pachytene spermatocytes. Bar in (C) represents 10 μm and applies to all panels.

Reference

PMID 25768017

Copyright

© 2015 Pacheco et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Fig 1. http://dx.doi.org/10.1371/journal.pgen.1005017.g001