File:Mouse E9.5 neural crest beta actin KO.jpg

Figure 2. At E9.5 Actb−/− embryos show defects in neural crest ontogeny. (A) Crabp1 whole mount and section ISH (at the indicated planes: a, b, c and d) on Actb+/+ and Actb−/− embryos. For the latter all sections show absence of neural crest cells in certain regions of the sections. Actb−/− neural crest cells are not able to reach the brachial arches (arrows in section d). (B) Sox10 whole mount embryo ISH marks the migrating neural crest. Sox10 ISH shows comparable results as the Crabp1 probe. (C) Pax3 marks the dermomyotome and the cranial neural crest. Compared to Actb+/+ embryos, the Actb−/− embryos show a similar expression pattern of pax3 at the level of the dermomyotomes, but a reduction in the cranial region. Panels depicting Actb+/+ and Actb−/− embryos are of the same magnification. doi:10.1371/journal.pone.0085608.g002

Reference

Beta-actin is required for proper mouse neural crest ontogeny. Tondeleir D, Noelanders R, Bakkali K, Ampe C. PLoS One. 2014 Jan 7;9(1):e85608. doi: 10.1371/journal.pone.0085608. eCollection 2014. PMID 24409333

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0085608

Journal.pone.0085608.g002.jpg

Copyright

© 2014 Tondeleir et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.