File:Reconstructed rat embryos 05.png

Left panels illustrate parthenogenic and reconstructed embryo activation using the calcium ionophore ionomycin followed by bohemine treatment.

There were no apparent differences between oocytes treated with 50 µM versus 100 µM bohemine, and activated parthenotes were indistinguishable from activated reconstructed rat embryos (RERs).

Right panels illustrate the development of rat embryos in mR1ECM medium. Less than 5% of rat embryos develop beyond the 4-cell stage, with only ~1% developing to the blastocyst stage (bottom panel, insert: arrow indicates blastocyst inner cell mass).

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http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0009799

Citation: Webb RL, Findlay KA, Green MA, Beckett TL, Murphy MP (2010) Efficient Activation of Reconstructed Rat Embryos by Cyclin-Dependent Kinase Inhibitors. PLoS ONE 5(3): e9799. doi:10.1371/journal.pone.0009799

Editor: Mark R. Cookson, National Institutes of Health, United States of America

Received: January 29, 2010; Accepted: March 1, 2010; Published: March 19, 2010

Efficient activation of reconstructed rat embryos by cyclin-dependent kinase inhibitors. Webb RL, Findlay KA, Green MA, Beckett TL, Murphy MP. PLoS One. 2010 Mar 19;5(3):e9799. PMID: 20333307

Copyright: © 2010 Webb et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.