File:Mice cloned from adult keratinocytes.jpg
Mice_cloned_from_adult_keratinocytes.jpg (600 × 398 pixels, file size: 20 KB, MIME type: image/jpeg)
Mice cloned from adult skin keratinocytes and other cells by NT.
(A) Cloned blastocysts from oocytes reconstructed by nuclear transfer (NT) of bulge follicle keratinocyte nuclei into unfertilized mouse oocytes. The α6+CD34+ bulge keratinocytes were isolated by FACS from skin of adult female K14-Cre/ROSA-floxed-lacZ mice and used directly in NT.
(B) Three mice cloned from the same donor mouse, an adult female. The pup on the left is from an α6+CD34+ bulge follicle keratinocyte nucleus, and the two pups on the right are from CC nuclei.
(C) Three mice cloned from the same donor mouse, an adult male. The pup on the left is from an α6+CD34+ bulge follicle keratinocyte nucleus, and the two pups on the right are from α6+CD34− keratinocyte nuclei.
(D) Six-week-old mice cloned by NT from α6+CD34+ bulge follicle keratinocytes isolated from an adult male.
Proc Natl Acad Sci U S A. 2007 February 20; 104(8): 2738–2743. Published online 2007 February 13. doi: 10.1073/pnas.0611358104.
Copyright © 2007 by The National Academy of Sciences of the USA
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1815251/?tool=pubmed
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| Gwalagizhi | Nyangagi | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 14:20, 24 February 2010 | | 600 × 398 (20 KB) | S8600021 (talk | contribs) | Mice cloned from adult skin keratinocytes and other cells by NT. (A) Cloned blastocysts from oocytes reconstructed by nuclear transfer (NT) of bulge follicle keratinocyte nuclei into unfertilized mouse oocytes. The α6+CD34+ bulge keratinocytes were iso |
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