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- 10:13, 12 April 2013 Z8600021 talk contribs uploaded File:Rugh 044.jpg (==Isolated germinal vesicle (nucleus) of Rami pipiens== Nucleus isolated in calcium-free Ringer's solution, from stage 6, showing sac-like organelles which protrude from the nuclear membrane. The cloud of central nucleoli surrounds and obscures the ch...)