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- 09:32, 28 April 2010 S8600021 talk contribs uploaded File:Mouse- axial skeleton intervertebral disc.jpg (Mouse- axial skeleton intervertebral disc Verification of localization by in situ hybridization. (A) A digoxigenin labelled probe to Nfatc1 was hybridized to sections from E12.5 day embryos. Hybridization was visualized as purple staining. Nfatc1 was c)